Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids

Autor: Marlène Perignon, Michel Pina, Camille Simonneau-Deve, Pierre Villeneuve, Anne Renault, Jérôme Lecomte
Přispěvatelé: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), St Hubert, St Hubert company in France
Rok vydání: 2011
Předmět:
Estérase
C-13
030309 nutrition & dietetics
[SDV]Life Sciences [q-bio]
General Chemical Engineering
Hydrolyse enzymatique
REGIOSPECIFIC ANALYSIS
Rhizopus oryzae
Regiodistribution
01 natural sciences
Esterase
03 medical and health sciences
chemistry.chemical_compound
Hydrolysis
Q02 - Traitement et conservation des produits alimentaires
Triglycéride
Typoselectivity
CHEMOENZYMATIC SYNTHESIS
Lipase
Q04 - Composition des produits alimentaires
0303 health sciences
Chromatography
biology
Triglyceride
010405 organic chemistry
Chemistry
Organic Chemistry
000 - Autres thèmes
Rhizopus oryzae lipase
Substrate (chemistry)
biology.organism_classification
0104 chemical sciences
Acide gras
Position analysis
Homogeneous
STRUCTURED TRIACYLGLYCEROLS
biology.protein
ACYL MIGRATION KINETICS
Activité enzymatique
TRIGLYCERIDES
Zdroj: Journal of the American Oil Chemists' Society
Journal of the American Oil Chemists' Society, Springer Verlag, 2012, 89 (1), pp.89-96. ⟨10.1007/s11746-011-1884-y⟩
ISSN: 1558-9331
0003-021X
DOI: 10.1007/s11746-011-1884-y
Popis: The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn -2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids.
Databáze: OpenAIRE
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