Lung Cyclic 3′,5′-Adenosine Monophosphate and Adenylate Cyclase in Endotoxic Shock
| Autor: | Diane M. Klein, Mohammed M. Sayeed |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Adenosine monophosphate medicine.medical_specialty Guinea Pigs Clinical Biochemistry Adenylate kinase Cyclase chemistry.chemical_compound In vivo Internal medicine Cyclic AMP medicine Animals Lung Molecular Biology chemistry.chemical_classification Chemistry Substrate (chemistry) Shock Septic Endocrinology Enzyme medicine.anatomical_structure Salmonella enteritidis Cyclase activity Adenylyl Cyclases |
| Zdroj: | Experimental Lung Research. 1:191-199 |
| ISSN: | 1521-0499 0190-2148 |
| Popis: | Cyclic 3',5'-adenosine monophosphate (cyclic AMP) and adenylate cyclase activity were measured in lungs from guinea pigs in endotoxic shock induced by an intravenous injection of Salmonella enteritidis endotoxin (10 mg/kg body weight). Both cyclic AMP content and adenylate cyclase activity were significantly elevated in lung tissue from the endotoxic guinea pigs. There was no apparent change in the affinity of adenylate cyclase for its substrate (ATP); however, the maximum velocity of the enzyme reaction was increased in lungs from the endotoxic group. Endotoxin, in the concentration range of 10(-4) to 10(-2) micrograms/ml, added to lung homogenates did not affect adenylate cyclase activity. Prostaglandins do not seem to mediate the effects of endotoxin in vivo on lung cyclic AMP since treatment of guinea pigs with indomethacin (10 mg/kg body weight) 30 min prior to endotoxin administration did not alter the endotoxin-induced increase in lung cyclic AMP. |
| Databáze: | OpenAIRE |
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