Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT
| Autor: | Peng Wang, Mengzhu Xue, Shaowei Du, Xin Hu, Haiyue Liu, Ke Zhang, Luonan Chen, Guoyi Jia, Lingshuang Wu, Yuwei Liu, Wanwan Feng, Liwen Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Science Gene regulatory network General Physics and Astronomy 02 engineering and technology Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Downregulation and upregulation Transcription (biology) microRNA Epithelial–mesenchymal transition lcsh:Science Regulation of gene expression Multidisciplinary Competing endogenous RNA Chemistry General Chemistry 021001 nanoscience & nanotechnology eye diseases Cell biology 030104 developmental biology embryonic structures lcsh:Q 0210 nano-technology TGFBI |
| Zdroj: | Nature Communications Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-09649-1 |
| Popis: | The competitive endogenous RNA (ceRNA) hypothesis suggests an intrinsic mechanism to regulate biological processes. However, whether the dynamic changes of ceRNAs can modulate miRNA activities remains controversial. Here, we examine the dynamics of ceRNAs during TGF-β-induced epithelial-to-mesenchymal transition (EMT). We observe that TGFBI, a transcript highly induced during EMT in A549 cells, acts as the ceRNA for miR-21 to modulate EMT. We further identify FN1 as the ceRNA for miR-200c in the canonical SNAIL-ZEB-miR200 circuit in MCF10A cells. Experimental assays and computational simulations demonstrate that the dynamically induced ceRNAs are directly coupled with the canonical double negative feedback loops and are critical to the induction of EMT. These results help to establish the relevance of ceRNA in cancer EMT and suggest that ceRNA is an intrinsic component of the EMT regulatory circuit and may represent a potential target to disrupt EMT during tumorigenesis. Competitive endogenous RNAs help to regulate biological processes by regulating miRNA activity levels. Here the author show TGFBI acts as a ceRNA for miR-21 in the epithelial-to-mesenchymal transition. |
| Databáze: | OpenAIRE |
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