Chronic restraint stress increases angiotensin II potency in the rat carotid: role of cyclooxygenases and reactive oxygen species

Autor: Katia Colombo Marchi, Carlos R. Tirapelli, Mayara S. Gomes, Hariane Côco, Thiago M. Cunha, Larissa Pernomian, Priscila Cristina Pereira, Alexandre H. Lopes, Ana Maria de Oliveira
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Contraction (grammar)
Pharmaceutical Science
Prostacyclin
030204 cardiovascular system & hematology
Muscle
Smooth
Vascular
Wortmannin
Phosphatidylinositol 3-Kinases
chemistry.chemical_compound
0302 clinical medicine
chemistry.chemical_classification
Oxadiazoles
biology
Angiotensin II
Catalase
Carotid Arteries
NADPH Oxidase 4
cardiovascular system
Muscle Contraction
medicine.drug
Restraint
Physical
medicine.medical_specialty
6-Ketoprostaglandin F1 alpha
Superoxide dismutase
03 medical and health sciences
Internal medicine
Renin–angiotensin system
medicine
Animals
Cyclooxygenase Inhibitors
Rats
Wistar
Pharmacology
Reactive oxygen species
business.industry
NADPH Oxidases
Hydrogen Peroxide
ESPÉCIES REATIVAS DE OXIGÊNIO
030104 developmental biology
Endocrinology
chemistry
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Vasoconstriction
Cyclooxygenase 1
biology.protein
Endothelium
Vascular
Corticosterone
Reactive Oxygen Species
business
Proto-Oncogene Proteins c-akt
Stress
Psychological
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 2042-7158
0022-3573
DOI: 10.1111/jphp.12659
Popis: ObjectivesTo investigate the mechanisms underlying the effects of chronic restraint stress on the vascular contractile response induced by angiotensin (Ang) II in rat carotid.MethodsConcentration–response curves for AngII were obtained in endothelium-intact or endothelium-denuded carotid rings, in the absence or presence of SC-560 (COX-1 inhibitor), SC-236 (COX-2 inhibitor), wortmannin (PI3K-Akt inhibitor), ML171 (NOX-1 inhibitor), VAS2870 (NOX-4 inhibitor), tiron (O2− scavenger) or PEG-catalase (H2O2 scavenger). 6-ketoPGF1α, TXB2, O2− or H2O2 levels and superoxide dismutase and catalase activity or expression were also measured in rat carotid.Key findingsStress increased AngII potency in rat carotid. Muscular COX-1 or COX-2-derived metabolites negatively modulated AngII-induced contraction in control rat carotid. Endothelial COX-1 or COX-2-derived metabolites positively modulated AngII-induced contraction in stressed rat carotid. PI3K-Akt, NOX-1, NOX-4, O2− and H2O2 positively modulated AngII-induced contraction in stressed rat carotid. Stress increased 6-ketoPGF1α or H2O2 generation and reduced catalase activity in rat carotid. Protein expression of COX-1, NOX-4 or p-Akt was increased in stressed rat carotid.ConclusionsStress increases AngII potency in rat carotid by a mechanism that involves the increased generation of PGI2 and H2O2 and the activation of Akt pathway. Such mechanism could play a pathophysiological role in cardiovascular diseases correlated with stress.
Databáze: OpenAIRE
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