Functional Expression and Cellular mRNA Localization of a G Protein-Activated K+inward Rectifier Isolated from Rat Brain
| Autor: | E. Dissmann, A. Spauschus, Christine Karschin, D. von Pfeil, Andreas Karschin, Erhard Wischmeyer |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Potassium Channels Structural similarity G protein Xenopus Protein subunit Molecular Sequence Data Biophysics Transfection Biochemistry Cell Line Open Reading Frames GTP-Binding Proteins Chlorocebus aethiops Animals Amino Acid Sequence RNA Messenger G protein-coupled inwardly-rectifying potassium channel Potassium Channels Inwardly Rectifying Rats Wistar Receptor Molecular Biology In Situ Hybridization Gene Library Base Sequence Sequence Homology Amino Acid biology Inward-rectifier potassium ion channel cDNA library Brain Cell Biology biology.organism_classification Molecular biology Recombinant Proteins Rats Cell biology G Protein-Coupled Inwardly-Rectifying Potassium Channels Oocytes Female |
| Zdroj: | Biochemical and Biophysical Research Communications. 223:474-479 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.0918 |
| Popis: | We have cloned by homology screening from a rat brain cDNA library a GIRK3-type (Kir 3.3) inwardly rectifying K + channel subunit with high structural similarity to other subfamily members whose activity is thought to be controlled by receptor-stimulated G proteins. When heterologously expressed both in Xenopus oocytes and in mammalian COS-7 cells, rbGIRK3 subunits individually fail to form functional channels. In contrast, when coexpressed with other GIRK subunits, rbGIRK3 gives rise to prominent currents which are enhanced by the stimulation of coexpressed 5-HT 1A receptors. In-situ hybridizations show that of all GIRK subunits rbGIRK3 is most widely distributed and strongly expressed throughout the rat brain and thus may play an important role in central signal processing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect