DNA methylation status of imprinted H19 and KvDMR1 genes in human placentas after conception using assisted reproductive technology
| Autor: | Xiaoming Teng, Kunming Li, Fengli Chi, Mei Zhao, Yingya Li, An-Qi Lin |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pregnancy 030219 obstetrics & reproductive medicine Assisted reproductive technology In vitro fertilisation medicine.medical_treatment General Medicine Reproductive technology Methylation Biology medicine.disease Intracytoplasmic sperm injection Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine embryonic structures DNA methylation medicine Original Article Genomic imprinting |
| Zdroj: | Ann Transl Med |
| ISSN: | 2305-5847 2305-5839 |
| DOI: | 10.21037/atm-20-3364 |
| Popis: | Background Assisted reproductive technologies (ARTs), such as in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), are thought to destabilize genomic imprints. Previous studies examining the association between ART and aberrant DNA methylation have been inconclusive. Method The DNA methylation status of H19 and KvDMR1was compared between newborns conceived through ART and those conceived naturally to evaluate the safety of ART. Placental tissues from 6 full-term, naturally conceived pregnancies (no gestational comorbidities) and six full-term ART pregnancies (no gestational complication) were collected. Genomic DNA (gDNA) and RNA were extracted from both groups. Real-time PCR was used to analyze the mRNA expression levels of H19 and KvDMR1 in the placenta for both groups. A whole-genome DNA methylation microarray was used to examine three placentas from full-term, naturally conceived pregnancies and three placentas from full-term IVF pregnancies. Result The expression level of H19 in the IVF group was significantly higher than that in the natural pregnancy group, whereas the expression level of KvDMR1 was significantly lower in the ART group than in the natural pregnancy group. Also, human ART manipulation resulted in placental gDNA methylation modifications. Conclusion: Abnormal methylation patterns were detected in phenotypically normal phenotype conceived by ART, which may occur due to imprinting errors in sperm/oocyte cells or side effects of in vitro embryo culture procedures. Further investigation is necessary to determine whether imprinted gene expression and DNA methylation can be regulated through other mechanisms. Keywords Assisted reproductive technology (ART); placenta; methylation; H19; KvDMR1. |
| Databáze: | OpenAIRE |
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