Antibodies against retroviral proteins and nuclear antigens in a subset of idiopathic CD4+ T lymphocytopenia patients
| Autor: | Cesar D. Fermin, Peter F. Kohler, Robert F. Garry, Hong Luo, M. Louise Markert |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Immunology Immunoblotting Retroviridae Proteins macromolecular substances medicine.disease_cause Antibodies Viral Autoantigens Virus Autoimmunity Immunodeficiency Syndrome Antigen Cytopathogenic Effect Viral Virology medicine Humans T-Lymphocytopenia Idiopathic CD4-Positive biology business.industry Virion Nuclear Proteins Antigens Nuclear T lymphocyte medicine.disease Coculture Techniques Cell nucleus Infectious Diseases medicine.anatomical_structure Antibodies Antinuclear biology.protein Lymphocytopenia Antibody business |
| Zdroj: | AIDS research and human retroviruses. 12(10) |
| ISSN: | 0889-2229 |
| Popis: | Idiopathic CD4+ T lymphocytopenia (ICL) is an immunodeficiency syndrome characterized by severe depletion of CD4+ T lymphocytes, but in which human immunodeficiency virus cannot be detected. Peripheral blood mononuclear cells (BPMCs) from an ICL patient were cocultured with HUT78 T-lymphoblastoid cells, and an acute cytopathic effect and formation of multinucleated cells were observed. A human intracisternal A-type retroviral particle designated HIAP-II was detected in cells surviving the acute cytopathic effect. Eight of 13 ICL patients in a blinded screen of a serological panel provided by the National Centers for Disease Control and Prevention (CDC) had serum antibodies that specifically reacted with HIAP-II associated proteins by Western immunoblotting. None of 19 control sera in the panel that were unreactive with HIV Gag proteins produced a positive result on HIAP-II immunoblots. Comparable results were obtained in a blinded screen of a second CDC serological panel. Sera from 8 of 14 ICL patients in the second serological panel were positive for antinuclear autoantibodies (ANAs) commonly observed in patients with systemic autoimmune diseases. These results suggest the possible involvement of an A-type retrovirus or autoimmunity in development of ICL in a subset of patients. |
| Databáze: | OpenAIRE |
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