Quantitative analysis of chemoresistance-inducing fatty acid in food supplements using UHPLC-ESI-MS/MS
| Autor: | Jörg Schlotterbeck, Agnes Kolb, Malgorzata Cebo, Michael Lämmerhofer |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Electrospray ionization
02 engineering and technology Mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry Fish Oils Limit of Detection Tandem Mass Spectrometry Sample preparation Data-independent acquisition chemistry.chemical_classification Chromatography Chemistry 010401 analytical chemistry Fatty Acids Fatty acid Reproducibility of Results 021001 nanoscience & nanotechnology Fish oil 0104 chemical sciences Drug Resistance Neoplasm Dietary Supplements 0210 nano-technology Quantitative analysis (chemistry) Food Analysis Polyunsaturated fatty acid Chromatography Liquid |
| Zdroj: | Analytical and bioanalytical chemistry. 411(2) |
| ISSN: | 1618-2650 |
| Popis: | Polyunsaturated fatty acids are important signaling molecules. A recent study reported hexadeca-4Z,7Z,10Z,13Z-tetraenoic acid, 12-oxo-5Z,8E,10E-heptadecatrienoic acid, and (12S)-hydroxy-5Z,8E,10E-heptadecatrienoic acid as chemotherapy resistance-inducing factors when tumor cells were treated with cisplatin. Marine-based food supplements like fish oil or algae extracts are rich in polyunsaturated fatty acids and can contain large amounts of hexadeca-4Z,7Z,10Z,13Z-tetraenoic acid. Thus, it was concluded that oral uptake of hexadeca-4Z,7Z,10Z,13Z-tetraenoic acid might induce chemoresistance as shown in a mouse model. Cancer patients tend to consume food supplements containing polyunsaturated fatty acids on a regular basis. The uptake of hexadeca-4Z,7Z,10Z,13Z-tetraenoic acid and (12S)-hydroxy-5Z,8E,10E-heptadecatrienoic acid should be controlled, because even low concentrations of 0.5 ng mL-1 showed chemoresistance-inducing effects in animal experiments. For accurate analysis of hexadeca-4Z,7Z,10Z,13Z-tetraenoic acid and (12S)-hydroxy-5Z,8E,10E-heptadecatrienoic acid a validated method was developed by using ultrahigh-performance liquid chromatography hyphenated to quadrupole time of flight mass spectrometry via electrospray ionization and sample preparation by solid-phase extraction (SPE) with 3-aminopropyl silica. A combined targeted/untargeted approach was utilized using MS/MS by data-independent acquisition with SWATH and applied to commercial food supplements (refined fish oil, fish oil capsules, algae oil capsules, and flaxseed capsules). Accurate quantification of hexadeca-4Z,7Z,10Z,13Z-tetraenoic acid and (12S)-hydroxy-5Z,8E,10E-heptadecatrienoic acid on the MS/MS level with simultaneous untargeted fatty acid screening revealed additional information. The LODs for hexadeca-4Z,7Z,10Z,13Z-tetraenoic acid and (12S)-hydroxy-5Z,8E,10E-heptadecatrienoic acid were 0.036 ng mL-1 and 0.054 ng mL-1, respectively. Since hexadeca-4Z,7Z,10Z,13Z-tetraenoic acid was present in the samples in large amounts and (12S)-hydroxy-5Z,8E,10E-heptadecatrienoic was not expected to be present in high concentrations, two calibration ranges, namely, 0.5–20 ng mL-1 and 5–200 ng mL-1, were validated. An untargeted screening identified 18–39 free fatty acids being present in the lipid extracts of the food supplement samples. |
| Databáze: | OpenAIRE |
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