Protection of Differentiating Neuronal Cells from Amyloid β Peptide-induced Injury by Alkaline Extract of Leaves of Sasa senanensis Rehder
| Autor: | Satoshi Yokose, Tomoyuki Abe, Takenori Natori, Soichi Iwama, Takaaki Oizumi, Toshikazu Yasui, Mineko Tomomura, Misaki Horiuchi, Yoshiko Masuda, Katsuji Oguchi, Mika Nakagawa, Mayumi Tsuji, Hiroshi Sakagami, Hiroshi Takeshima, Akito Tomomura, Nobuaki Tamura, Tomohiro Fujisawa, Hiroshi Oizumi, Yuji Kiuchi, Hayato Suzuki, Kenta Tanaka |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Survival Cellular differentiation Cell Stimulation Protein Aggregation Pathological Neuroprotection Antioxidants General Biochemistry Genetics and Molecular Biology Protein Aggregates 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Animals Humans Viability assay Cytotoxicity Neurons Pharmacology Amyloid beta-Peptides Plant Extracts Chemistry Cell Differentiation Molecular biology Rats Plant Leaves Neuroprotective Agents 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Toxicity Sasa Keratinocyte Research Article |
| Zdroj: | In Vivo. 32 |
| ISSN: | 1791-7549 |
| DOI: | 10.21873/invivo.11229 |
| Popis: | Background/aim We have previously reported the protection of doxorubicin-induced keratinocyte toxicity by alkaline extract of the leaves of Sasa senanensis Rehder (SE). In order to extend the generality of the cell protective effect of SE, we investigated whether it also protects rat PC12 and human SH-SY5Y neuron model cells from amyloid β-peptide (Aβ)-induced injury. Materials and methods Viability of cells was determined by the MTT method. Cytotoxicity was evaluated by the concentration that reduces the cell viability by 50% (CC50). Protection from Aβ-induced cytotoxicity was evaluated by the concentration that reversed the Aβ-induced reduction of viability by 50% (EC50). The selectivity index (SI) of neuroprotective activity was defined as the ratio of EC50 to CC50 Aβ1-42 aggregation was assayed using Aβ1-42 ammonium hydroxide. Results SE showed hormetic growth stimulation at lower concentrations in both neuron precursors and differentiated cells. SE reproducibly inhibited Aβ-induced cytotoxicity against both undifferentiated and differentiated neuron cells. Both the extent of differentiation induction and viability depended on the cell density, suggesting the release of growth and differentiation stimulation substances into culture supernatant. Higher concentrations of SE partially reduced the Aβ1-42 aggregation. Conclusion Hormetic growth stimulation and inhibition of aggregation may be involved in the neuroprotective activity of SE. |
| Databáze: | OpenAIRE |
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