Reproducible insulin secretion from isolated rat pancreas preparations using an organ bath
| Autor: | Keitaro Satoh, Tomoe Fujita, Asuka Morita, Motoshi Ouchi, Ken-ichi Inoue, Naohiko Anzai, Hiroe Kon, Keitaro Hayashi, Naoyuki Otani, Satoko Kishimoto, Misao Terada |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty amylase insulin Original medicine.medical_treatment 030209 endocrinology & metabolism In Vitro Techniques General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Organ Culture Techniques Glucagon-Like Peptide 1 Internal medicine Diabetes mellitus Insulin-Secreting Cells Insulin Secretion medicine Animals Secretion Amylase Rats Wistar Incubation Pancreas General Veterinary biology organ bath Chemistry Insulin rat pancreas Reproducibility of Results General Medicine medicine.disease Glucagon-like peptide-1 030104 developmental biology medicine.anatomical_structure Endocrinology glucagon-like peptide-1 Amylases biology.protein Animal Science and Zoology Ex vivo |
| Zdroj: | Experimental Animals |
| ISSN: | 1881-7122 |
| Popis: | Diabetes mellitus is a lifestyle-related disease that is characterized by inappropriate or diminished insulin secretion. Ex vivo pharmacological studies of hypoglycemic agents are often conducted using perfused pancreatic preparations. Pancreas preparations for organ bath experiments do not require cannulation and are therefore less complex than isolated perfused pancreas preparations. However, previous research has generated almost no data on insulin secretion from pancreas preparations using organ bath preparations. The purpose of this study was to investigate the applicability of isolated rat pancreas preparations using the organ bath technique in the quantitative analysis of insulin secretion from β-cells. We found that insulin secretion significantly declined during incubation in the organ bath, whereas it was maintained in the presence of 1 µM GLP-1. Conversely, amylase secretion exhibited a modest increase during incubation and was not altered in the presence of GLP-1. These results demonstrate that the pancreatic organ bath preparation is a sensitive and reproducible method for the ex vivo assessment of the pharmacological properties of hypoglycemic agents. |
| Databáze: | OpenAIRE |
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