Multidrug-resistant Raoultella ornithinolytica misidentified as Klebsiella oxytoca carrying blaOXA β-lactamases: antimicrobial profile and genomic characterization
| Autor: | Juan Carlos Bravata-Alcántara, Noé Escobar-Escamilla, Fabiola Garcés-Ayala, Juan Manuel Bello-López, Adnan Araíza Rodriguez, Julio Cesar Juárez-Gómez, Víctor Hugo Gutiérrez-Muñoz, Iliana Alejandra Cortés-Ortíz, Edgar Mendieta-Condado, José Ernesto Ramírez–González |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.drug_class
Cephalosporin Microbial Sensitivity Tests Biochemistry Microbiology beta-Lactamases Antibiotic resistance Bacterial Proteins Enterobacteriaceae polycyclic compounds Genetics medicine Molecular Biology biology Klebsiella oxytoca General Medicine Genomics biochemical phenomena metabolism and nutrition Plasmid-mediated resistance biology.organism_classification Antimicrobial Raoultella ornithinolytica Anti-Bacterial Agents Penicillin Multiple drug resistance bacteria medicine.drug |
| Zdroj: | Archives of microbiology. 203(9) |
| ISSN: | 1432-072X |
| Popis: | Class D β-lactamases OXA-232 and OXA-48 hydrolyze penicillin, cephalosporins and carbapenems, limiting the pharmacological therapeutics in bacteraemia. OXA producer microorganisms are considered a great emergent threat, especially in nosocomial environments. To determine the resistance profile and genomic characterization of two isolates initially identified as potential carbapenemase-producer Klebsiella oxytoca in a third level hospital. Automated platform BD Phoenix-100 System was used to identify and to biochemically characterize both isolates. Furthermore, the resistance profile was determined through CLSI methods and the whole genome sequences were obtained using Next-Generation Sequencing. Resistance genes were analyzed, and the virtual fingerprinting was determined to corroborate the similarity with related bacteria. Both strains correspond to Raoultella ornithinolytica carrying OXA 232 and OXA-48 genes, confirming the class D β-lactamases assay results. Here, we present the genetic and phenotypic analysis of multidrug resistance R. ornithinolytica, representing the first report in Mexico. |
| Databáze: | OpenAIRE |
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