MRP8/14 does not contribute to dissemination or inflammation in a murine model of Lyme borreliosis
| Autor: | Thomas Vogl, Lauren M.K. Mason, Jasmin I. Ersoz, Joppe W. Hovius, Anneke Oei, Jeroen Coumou, Tom van der Poll, Joris J. T. H. Roelofs |
|---|---|
| Přispěvatelé: | AII - Infectious diseases, Experimental Immunology, Graduate School, ACS - Pulmonary hypertension & thrombosis, Pathology, ACS - Diabetes & metabolism, Center of Experimental and Molecular Medicine, 01 Internal and external specialisms, Infectious diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Neutrophils medicine.medical_treatment Immunology Inflammation Biology Lyme Arthritis Monocytes Microbiology Mice 03 medical and health sciences Immune system Phagocytosis Borrelia medicine Animals Calgranulin B Humans Immunology and Allergy Calgranulin A Borrelia burgdorferi Mice Knockout Lyme Disease Hematology biology.organism_classification bacterial infections and mycoses Immunity Innate LYME Mice Inbred C57BL Disease Models Animal 030104 developmental biology Cytokine biology.protein bacteria Female medicine.symptom Antibody |
| Zdroj: | Immunobiology, 223(11), 694-698. Urban und Fischer Verlag GmbH und Co. KG |
| ISSN: | 0171-2985 |
| Popis: | Myeloid-related protein (MRP)8 and MRP14 form a complex (MRP8/14) that is released by activated neutrophils and monocytes during infection. MRP8/14 has been shown to have bacteriostatic activity in vitro against Borrelia burgdorferi, the spirochete that causes Lyme borreliosis. Furthermore, levels of MRP8/14 have been shown to be elevated in the joints of patients with Lyme arthritis. We hypothesized that MRP8/14 has a protective effect during B. burgdorferi infection. To determine the role of MRP8/14 in the immune response to B. burgdorferi, we studied the course of B. burgdorferi infection in wildtype (wt) and mrp14−/− mice. In addition, we studied the response of leukocytes from mice lacking MRP8/14 to B. burgdorferi ex vivo. We demonstrated similar levels of B. burgdorferi dissemination, cytokine and immunoglobulin production in infected wt and mrp14−/− mice after 21 days. Neutrophils and monocytes lacking MRP8/14 were undiminished in their ability to become activated or phagocytose B. burgdorferi. In conclusion, we did not find a central role of MRP8/14 in the immune response against B. burgdorferi. As the levels of MRP8/14 in the serum of infected mice were low, we speculate that MRP8/14 is not released in levels great enough to influence the course of B. burgdorferi infection. |
| Databáze: | OpenAIRE |
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