Ciliogenesis in normal human kidney development and post-natal life
| Autor: | Mirna Saraga-Babić, Marijan Saraga, Katarina Vukojević, Ivana Bočina, Kristina Drnasin |
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| Rok vydání: | 2011 |
| Předmět: |
Morphogenesis
Lumen (anatomy) Kidney development Fluorescent Antibody Technique Gestational Age Biology Kidney Tubulin Ciliogenesis Cell polarity medicine Humans Cilia Cell Proliferation Tight junction human embryo kidney development primary cilia ɣ-tubulin Oct-4 urogenital system Cilium Age Factors Cell Polarity Nephrons Cell biology Microscopy Electron medicine.anatomical_structure Ki-67 Antigen Kidney Tubules Nephrology Child Preschool Pediatrics Perinatology and Child Health Octamer Transcription Factor-3 |
| Zdroj: | Pediatric nephrology (Berlin, Germany). 27(1) |
| ISSN: | 1432-198X |
| Popis: | Ciliogenesis in developing and post-natal human kidneys appears to influence cell proliferation and differentiation, apico-basal cell polarity, and tubular lumen formation. We have analyzed the appearance of primary cilia and differentiation of kidney cells in ten human conceptuses aged 6-22 weeks and in one 5-year-old kidney, using a double immunofluorescence labeling technique for α-tubulin, γ-tubulin, Oct-4, and Ki-67 and by electron microscopy. Immature forms of nephrons and ampullae were characterized by intense cell proliferation, which subsequently decreased during development. Primary cilia appeared on the surfaces of non-proliferating cells in developing nephrons, gradually increasing in length from 0.59 μm in renal vesicles to 0.81 μm in the S-forms of nephrons, ultimately reaching 3.04 μm in length in mature fetal and post-natal nephrons. Ciliary length increased from 0.59 μm in ampullae to 1.28 μm in post-natal collecting tubules. Mesenchymal to epithelial transformation of kidney cells coincided with the appearance of apico-basal polarity, both gap and tight junctions, and lumen formation. Up-regulation of Oct-4 expression correlated with the onset of kidney cell differentiation. Our results demonstrate the importance of proper primary cilia lengthening and Oct-4 expression for the normal development of fetal and post-natal kidneys and of apico-basal polarity for normal tubular lumen formation. Disturbances in these processes are associated with ciliopathies. |
| Databáze: | OpenAIRE |
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