Disruption of Botryococcus braunii colonies by glycoside hydrolases
| Autor: | Amélie Saumonneau, Nathan Lagneau, Lydia Awuor Ogonda, Catherine Dupré, Stéphanie Dutertre, Dominique Grizeau, Charles Tellier, Cyrille Grandjean, Franck Daligault |
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| Přispěvatelé: | Unité en Sciences Biologiques et Biotechnologies de Nantes (US2B), Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), University of Nairobi Department of Biochemistry, School of Medicine, College of health sciences, Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - Institut Universitaire de Technologie Saint-Nazaire (Nantes Univ - IUT Saint-Nazaire), Centre de Microscopie de Rennes (MRic), Université de Rennes (UR)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), We acknowledge France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INBS-04).This work was funded by the University of Nantes within the interdisciplinary project framework program., ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010) |
| Rok vydání: | 2023 |
| Předmět: |
Botryococcus braunii
Environmental Engineering Renewable Energy Sustainability and the Environment [SDV]Life Sciences [q-bio] [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Bioengineering Flow cytometry Extracellular matrix Waste Management and Disposal Hydrocarbons Microalga Glycosidase |
| Zdroj: | Bioresource Technology Reports Bioresource Technology Reports, 2023, 21, pp.101335. ⟨10.1016/j.biteb.2023.101335⟩ |
| ISSN: | 2589-014X |
| DOI: | 10.1016/j.biteb.2023.101335 |
| Popis: | International audience; Microalgae are a promising alternative resource to fossil-based products. Botryococcus braunii is a colonial green microalga having the ability to convert CO2 by photosynthesis into long chain hydrocarbons. These are excreted and trapped in an extracellular matrix (ECM). A panel of glycosidases ranging from arabinanase, galactananase to endoglucanase was tested for their ability to lyse the polysaccharides maintaining the B. braunii colony integrity in order to release the hydrocarbons present in the extracellular matrix without harming the cells. The BpGH9 endoglucanase from Bacillus pumilus was fused with CtCBM3a from Clostridium thermocellum and yellow fluorescent protein to probe the presence of microcrystalline cellulose in the cell wall of B. braunii and to increase the efficacy of the endoglucanase. All the tested enzymes were able to some extent to dissociate the cells from the extracellular matrix while keeping them alive, suggesting the feasibility of a semi-continuous in situ recovery of hydrocarbons. |
| Databáze: | OpenAIRE |
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