Field-study screening of blood folate concentrations: specimen stability and finger-stick sampling
| Autor: | E W Gunter, A Davoren, S D O'Broin, B P Kelleher |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Blood Specimen Collection Nutrition and Dietetics Chromatography Red Cell Medicine (miscellaneous) Blood volume Venous blood Biology Fingers Blood cell Red blood cell Folic Acid medicine.anatomical_structure Biochemistry medicine Humans Regression Analysis Female Hemoglobin Whole blood Blood sampling |
| Zdroj: | The American Journal of Clinical Nutrition. 66:1398-1405 |
| ISSN: | 0002-9165 |
| DOI: | 10.1093/ajcn/66.6.1398 |
| Popis: | We describe optimized procedures for field studies of blood folate concentrations by using finger-stick blood sampling and include relevant studies on blood folate stability. We introduce whole-blood folate adjustment using sample hemoglobin (folate/hemoglobin, nmollg) as a novel and practical tool yielding accurate and precise results when blood volume or dilution is unknown. Red cell folate concentrations (nmollL) of 1 1 887 Americans correlated well with hemoglobin-corrected whole- blood folate concentrations (r� = 0.993; red cell folate = 0.347 X hemoglobin folate + I nmol/L), which supports the approach of using the mean cell hemoglobin concentration (g/L) to interconvert red cell and hemoglobin folate data. Folate concentrations in capillary (finger stick) and venous blood samples from 28 normal donors were similar (P > 0.87), correlating closely (r = 0.98, P < 0.001). Whole-blood samples (collected into K2-EDTA-contain- ing evacuated tubes) in field studies are best stored intact at 4 #{176}C until they can be processed and frozen (-20 #{176}C). Specific knowl- edge of blood folate stability is essential in planning and designing field studies. Am J Gum Nutr l997;66: I 398-405. |
| Databáze: | OpenAIRE |
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