Lesion of the subthalamic nucleus reverses motor deficits but not death of nigrostriatal dopaminergic neurons in a rat 6-hydroxydopamine-lesion model of Parkinson's disease

Autor: Janete Aparecida Anselmo-Franci, Francesca Matheussi, Ana Marcia Delattre, Anete Curte Ferraz, Raphael E. Szawka, Léder Leal Xavier, V. Rizelio, L. Saur, M. Meneses, Matilde Achaval, Paula Rigon
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Male
Tyrosine hydroxylase immunohistochemistry
Physiology
Dopamine
Parkinson's disease
Nigrostriatal pathway
Biochemistry
Subthalamic nucleus
Random Allocation
General Pharmacology
Toxicology and Pharmaceutics
Medial forebrain bundle
lcsh:QH301-705.5
Neurons
lcsh:R5-920
General Neuroscience
Dopaminergic
General Medicine
Immunohistochemistry
Substantia Nigra
medicine.anatomical_structure
Anesthesia
Pharmaceutical Vehicles
Doença de Parkinson
medicine.symptom
lcsh:Medicine (General)
6-Hydroxydopamine
medicine.drug
N-Methylaspartate
animal structures
Tyrosine 3-Monooxygenase
Immunology
Biophysics
Substantia nigra
Motor Activity
Lesion
Núcleo subtalâmico
medicine
Animals
Oxidopamina
Parkinson Disease
Secondary
Rats
Wistar
Pars compacta
business.industry
Cell Biology
Rats
Substantia nigra pars compacta
Substância negra
nervous system
lcsh:Biology (General)
Parkinson’s disease
business
Zdroj: Brazilian Journal of Medical and Biological Research, Vol 43, Iss 1, Pp 85-95 (2010)
Scopus-Elsevier
Brazilian Journal of Medical and Biological Research, Volume: 43, Issue: 1, Pages: 85-95, Published: JAN 2010
Repositório Institucional da UFRGS
Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
Brazilian Journal of Medical and Biological Research v.43 n.1 2010
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Popis: The objective of the present study was to determine whether lesion of the subthalamic nucleus (STN) promoted by N-methyl- D-aspartate (NMDA) would rescue nigrostriatal dopaminergic neurons after unilateral 6-hydroxydopamine (6-OHDA) injection into the medial forebrain bundle (MFB). Initially, 16 μg 6-OHDA (6-OHDA group) or vehicle (artificial cerebrospinal fluid - aCSF; Sham group) was infused into the right MFB of adult male Wistar rats. Fifteen days after surgery, the 6-OHDA and Sham groups were randomly subdivided and received ipsilateral injection of either 60 mM NMDA or aCSF in the right STN. Additionally, a control group was not submitted to stereotaxic surgery. Five groups of rats were studied: 6-OHDA/NMDA, 6-OHDA/Sham, Sham/ NMDA, Sham/Sham, and control. Fourteen days after injection of 6-OHDA, rats were submitted to the rotational test induced by apomorphine (0.1 mg/kg, ip) and to the open-field test. The same tests were performed again 14 days after NMDA-induced lesion of the STN. The STN lesion reduced the contralateral turns induced by apomorphine and blocked the progression of motor impairment in the open-field test in 6-OHDA-treated rats. However, lesion of the STN did not prevent the reduction of striatal concentrations of dopamine and metabolites or the number of nigrostriatal dopaminergic neurons after 6-OHDA lesion. Therefore, STN lesion is able to reverse motor deficits after severe 6-OHDA-induced lesion of the nigrostriatal pathway, but does not protect or rescue dopaminergic neurons in the substantia nigra pars compacta.
Databáze: OpenAIRE
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