Osteoclasts in the interface with electrospun hydroxyapatite
| Autor: | Mikko Ritala, Jenni Pasuri, Kyösti Kauppinen, Juha Tuukkanen, Eero Santala, Jani Holopainen, Hanna Kokkonen, Petri Lehenkari, Maria Persson |
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| Rok vydání: | 2015 |
| Předmět: |
Osteoclasts
Enzyme-Linked Immunosorbent Assay Cell morphology Cell Line Mice Colloid and Surface Chemistry Tissue engineering X-Ray Diffraction Osteoclast medicine Animals Humans Physical and Theoretical Chemistry Matrigel Chemistry Interleukin-6 Tumor Necrosis Factor-alpha Surfaces and Interfaces General Medicine Resorption Cell biology medicine.anatomical_structure Durapatite Microscopy Fluorescence Cell culture Nanofiber Immunology Microscopy Electron Scanning Cytokine secretion Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 135 |
| ISSN: | 1873-4367 |
| Popis: | Electrospinning is a method to produce lightweight, resorbable and bioinspired scaffolds for tissue engineering. Here we investigated the influence of electrospun hydroxyapatite fibers (HA) on macrophages and osteoclasts. A mouse macrophage cell line (RAW 264.7) and human bone marrow derived primary osteoclasts (hOC) were cultured with electrospun HA fibers embedded in Matrigel. Cell morphology and the secretion of pro-inflammatory cytokines (IL-6 and TNF-α) were analyzed using macrophages. Both fluorescent microscopy and scanning electron microscopy indicated that the cell morphology differed on the various materials (HA fibers on Matrigel, pure Matrigel and a glass control). Control macrophages were activated with bacterial lipopolysaccharide (LPS) but electrospun HA did not provoke an inflammatory response. Cytokine secretion detected with enzyme-linked immunosorbent assay (ELISA) also supported this observation. LPS, but not HA fibers, stimulated TNF-α and IL-6 secretion by macrophages at the 2 day time point. After 4 days in culture there was an increasing trend in cytokine secretion in the HA fiber samples. Human bone marrow myeloid precursor cells were able to fuse and differentiate on the fibrous mineral scaffold to form functional multinuclear osteoclasts that were able to resorb the HA nanofibers. This indicates that osteoclasts do not necessarily need a continuous bone surface but osteoclast ruffled border membranes can form a resorption interface with a fibrous mineral scaffold. |
| Databáze: | OpenAIRE |
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