Phosphatidylcholine and L-acetyl-carnitine-based freezing medium can replace egg yolk and preserves human sperm function
| Autor: | Viviane Paiva Santana, Rui Alberto Ferriani, A.A. Vireque, Fernanda Sicchieri, Aline Bomfim Silva, Maria Aparecida Carneiro Vasconcelos, Rosana Maria dos Reis |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
endocrine system 030219 obstetrics & reproductive medicine medicine.diagnostic_test Cryoprotectant Chemistry urogenital system Urology Motility Semen Semen analysis Sperm Cryopreservation Andrology 03 medical and health sciences 0302 clinical medicine Reproductive Medicine medicine DNA fragmentation TÉCNICAS DE REPRODUÇÃO ASSISTIDA Original Article Sperm motility 030304 developmental biology |
| Zdroj: | Transl Androl Urol Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2223-4691 |
| Popis: | BACKGROUND: Conventional cryopreservation methods induce chemical and mechanical damage to the sperm membranes. The cryoprotectant potential of phospholipids of vegetal origin as soybean lecithin has been investigated as a substitute for egg yolk in diluents used for the cryopreservation of human spermatozoa. Therefore, the objective of this study was comparing the efficacy of a synthetic cryoprotectant supplemented with L-α-phosphatidylcholine (PC) and L-acetyl-carnitine (ANTIOX-PC) and the standard egg-based TEST-yolk buffer (TYB) in preserving sperm motility and chromatin quality in cryopreserved semen samples. METHODS: Prospective experimental study in which semen samples from 63 men with normal sperm motility and 58 men with low sperm motility were included and analyzed both before and after cryopreservation using ANTIOX-PC or TYB freezing media. Sperm quality was evaluated by routine semen analysis and DNA fragmentation index using the Terminal deoxynucleotidyl transferase dUTP nick end labeling assay. RESULTS: Differences in the post-thaw progressive motility and DNA fragmentation index were not detected between TYB and ANTIOX-PC cryoprotectants in both normal and low sperm motility groups (P>0.05). However, ANTIOX-PC medium retained higher non-progressive motility and lower percentage of immotile sperm when compared to TYB medium, resulting in a greater total motile sperm count (P |
| Databáze: | OpenAIRE |
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