| Autor: |
Yuzawa, Tomohisa, Matsuoka, Misato, Sumitani, Megumi, Fugaku Aoki, Sezutsu, Hideki, Suzuki, Masataka G. |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.12986479.v1 |
| Popis: |
Additional file 5: Fig. S1. Generation of BmdsxM and BmdsxF knockout silkworm lines using TALENs. (A) The target sites of TALENs within the female-specific exon (exon 3) and the male-specific coding region in exon 5 are shown. The rectangles indicate exons. Exons 3 and 4 are skipped when Bmdsx pre-mRNA is spliced in males. The gray region encodes the female-specific open reading frame (ORF). The black region encodes the male-specific ORF. TAL effector-binding sequences are shown in blue, while spacer sequences are indicated in red. (B) The deletion mutations introduced in the BmdsxFΔ85 and BmdsxMΔ7 lines are shown in (B) and (C), respectively. The uppercase characters in (B) represent the nucleotide sequence of Bmdsx exon 3. The uppercase characters in (C) indicate the nucleotide sequence of Bmdsx exon 5. Colons indicate identical nucleotide sequences between wild-type and mutant animals. Spacer sequences are indicated in red. 5′ splice donor and 3′ splice acceptor sites are shown in bold characters. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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