The calmodulin-dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans
| Autor: | R L Means, R L Kincaid, C Garen, Anthony R. Means, S Brining, CD Rasmussen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Calmodulin
Protein subunit Genes Fungal Molecular Sequence Data Phosphatase Catalysis Aspergillus nidulans General Biochemistry Genetics and Molecular Biology Gene Expression Regulation Fungal Gene expression Phosphoprotein Phosphatases Amino Acid Sequence RNA Messenger Cloning Molecular DNA Fungal Molecular Biology Binding Sites Sequence Homology Amino Acid Base Sequence biology General Immunology and Microbiology Calcineurin General Neuroscience Cell Cycle RNA Fungal Sequence Analysis DNA biology.organism_classification Molecular biology Calmodulin-binding proteins Mutagenesis Insertional Mutagenesis Essential gene biology.protein Genes Lethal Calmodulin-Binding Proteins Sequence Alignment Research Article |
| Zdroj: | The EMBO Journal. 13:3917-3924 |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1994.tb06703.x |
| Popis: | The gene encoding the homologue of the catalytic subunit of the Ca2+/calmodulin-regulated protein phosphatase 2B (calcineurin A) has been isolated from Aspergillus nidulans. This gene, cnaA+, is essential in this fungal system. Analysis of growth-arrested cells following gene disruption by homologous recombination reveals that they are blocked early in the cell cycle. The cnaA+ gene encodes a 2.5 kb mRNA and the deduced protein sequence is highly homologous to the calcineurin A subunit of other species. The mRNA varies in a cell cycle-dependent manner with maximal levels found early in G1 and considerably before the G1/S boundary. As calmodulin is also essential for A. nidulans cell cycle progression and levels rise before the G1/S boundary, our data suggest that calcineurin may represent a primary target for calmodulin at this cell cycle transition point. |
| Databáze: | OpenAIRE |
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