Induction of differentiation of HL-60 cells along the monocytic pathway by 5-methyltetrahydrofolate
Autor: | Pa, Bernabei, Mini E, Gattei V, Fc, Agostino, Bezzini R, Saccardi R, VALERIA SANTINI, Coronnello M, Mazzei T, Rossi Ferrini P |
---|---|
Rok vydání: | 1989 |
Předmět: |
0301 basic medicine
Purine Cellular differentiation 030106 microbiology Monocytes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Leukemia Promyelocytic Acute HLA Antigens Tumor Cells Cultured Humans Pharmacology (medical) Tetrahydrofolates Pharmacology Chemistry Cell Cycle Cell Differentiation Metabolism Thymine Infectious Diseases Oncology Biochemistry Cell culture 030220 oncology & carcinogenesis DNA methylation Transmethylation DNA |
Zdroj: | Europe PubMed Central |
ISSN: | 1120-009X |
Popis: | Many compounds of different chemical structure can induce the HL-60 cell line to differentiate along the monocytic or granulocytic pathway, but the mechanism(s) of differentiation by these agents is not known. Experimental evidence suggests that DNA and/or membrane phospholipid transmethylation reactions may be of importance. Based on this background, we have studied the effects of various concentrations of (dl)-5-methyltetrahydrofolate (mTHF), a folate coenzyme involved in transmethylation reactions, on differentiation of HL-60 cells. Differentiation along the monocytic pathway was evidenced in kinetic, functional, cytochemical and immunophenotypical studies when cells were treated with high-dose (dl)-mTHF (1 x 10(-3)M). Some hypotheses on the mechanism(s) of (dl)-mTHF induced HL-60 cell differentiation are discussed with particular regard to a possible enhancement of lipid or DNA methylation via methionine formation by the (l) form or to inhibition of folate-dependent metabolism by the unnatural (d) form, hence of purine and thymine nucleotide synthesis. |
Databáze: | OpenAIRE |
Externí odkaz: |