Multimodal gadolinium oxysulfide nanoparticles: a versatile contrast agent for mesenchymal stem cell labeling

Autor: Marc Verelst, Julien Santelli, Robert Mauricot, Séverine Lechevallier, H. Baaziz, Marine Vincent, Cyril Martinez, Angelo Parini, Daniel Cussac
Přispěvatelé: Matériaux Multi-fonctionnels et Multi-échelles (CEMES-M3), Centre d'élaboration de matériaux et d'études structurales (CEMES), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA), Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut de médecine moléculaire de Rangueil (I2MR), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), ChromaLys S.A.S.
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Contrast Media
Gadolinium
02 engineering and technology
CHO Cells
Cell therapy
03 medical and health sciences
Mice
Cricetulus
Microscopy
Electron
Transmission
In vivo
medicine
Fluorescence microscope
Human Umbilical Vein Endothelial Cells
Animals
Humans
General Materials Science
Magnetite Nanoparticles
ComputingMilieux_MISCELLANEOUS
medicine.diagnostic_test
Mesenchymal stem cell
Magnetic resonance imaging
Cell Differentiation
Mesenchymal Stem Cells
[CHIM.MATE]Chemical Sciences/Material chemistry
021001 nanoscience & nanotechnology
Magnetic Resonance Imaging
In vitro
Rats
Mice
Inbred C57BL
030104 developmental biology
Microscopy
Fluorescence
Cell Tracking
Rats
Inbred Lew
Nanoparticles
Female
Rabbits
Stem cell
0210 nano-technology
Ex vivo
Biomedical engineering
HeLa Cells
Zdroj: Nanoscale
Nanoscale, Royal Society of Chemistry, 2018, 10 (35), pp.16775-16786. ⟨10.1039/C8NR03263G⟩
ISSN: 2040-3364
2040-3372
DOI: 10.1039/C8NR03263G⟩
Popis: Despite a clear development of innovative therapies based on stem cell manipulation, the availability of new tools to better understand and follow stem cell behavior and improve their biomedical applications is not adequate. Indeed, an ideal tracking device must have good ability to label stem cells as well as complete neutrality relative to their biology. Furthermore, preclinical studies imply in vitro and in vivo approaches that often require several kinds of labeling and/or detection procedures. Consequently, the multimodality concept presented in this work may present a solution to this problem as it has the potential to combine complementary imaging techniques. Spherical europium-doped gadolinium oxysulfide (Gd2O2S:Eu3+) nanoparticles are presented as a candidate as they are detectable by (1) magnetic resonance (MRI), (2) X-ray and (3) photoluminescence imaging. Whole body in vivo distribution, elimination and toxicity evaluation revealed a high tolerance of nanoparticles with a long-lasting MRI signal and slow hepatobiliary and renal clearance. In vitro labeling of a wide variety of cells unveils the nanoparticle potential for efficient and universal cell tracking. Emphasis on mesenchymal stromal cells (MSCs) leads to the definition of optimal conditions for labeling and tracking in the context of cell therapy: concentrations below 50 μg mL−1 and diameters between 170 and 300 nm. Viability, proliferation, migration and differentiation towards mesodermal lineages are preserved under these conditions, and cell labeling appears to be persistent and without any leakage. Ex vivo detection of as few as five thousand Gd2O2S:Eu3+-labeled MSCs by MRI combined with in vitro examination with fluorescence microscopy highlights the feasibility of cell tracking in cell therapy using this new nanoplatform.
Databáze: OpenAIRE
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