| Popis: |
Additional file 3: Figure S3. qRT-PCR analysis of selected T. pratense genes: A) tdn_146439/ENGase85A (encodes a cytosolic beta-endo-N-acetyglucosaminidase (ENGase), B) tdn_69411/LTP (Lipid transfer protein, a pathogenesis-related protein), C) tdn85889/PME44 (pectin methylesterase 44), D) K65_9861 P5CS (DELTA1-PYRROLINE-5-CARBOXYLATE SYNTHASE 1). Two additional T. pratense tissues (axial meristem (AM) and leaves (L)) were included and also the six RNA extractions also used for constructing the transcriptome libraries. Transcriptome data are marked with TPM, all samples are from mown (M) or not mown (NM) plants. Gene expression levels were normalized to the housekeeping contig k65_5754 (not annotated, but with similar expression throughout the transcriptomes). Shown are mean values of the fold change of the respective contig in relation to the expression of contig K65_5754 with error bars that represent standard deviation. On the right side of each graph, the log2 values of the respective gene in each transcriptome are shown for better comparison of expression patterns. |
