Harmonization of the quantitative determination of volatile fatty acids profile in aqueous matrix samples by direct injection using gas chromatography and high-performance liquid chromatography techniques: Multi-laboratory validation study
| Autor: | Joan Noguerol-Arias, David Bolzonella, Anthony D. Schuit, Angel F. Mohedano, Jesús A. Cacho, Cecilia Sambusiti, Rafael Borja, J.C. Frigon, Audrey Battimelli, Jana Muehlenberg, Jan Mumme, Francisco Sáez Raposo, Gustavo A. Peñuela |
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| Přispěvatelé: | UAM. Departamento de Química Física Aplicada |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
anaerobic digestion
Validation study Chromatography Gas high performance liquid chromatography gas chromatography limit of quantitation Aqueous matrix samples Analytical chemistry Biochemistry High-performance liquid chromatography Performance parameters Analytical Chemistry Quantitative determinations Matrix (chemical analysis) Aqueous matrices Volatile fatty acids Chromatographic techniques Validation Calibration Parameter estimation Fatty acids Chromatography High Pressure Liquid Detection limit Interlaboratory studies Interlaboratory study Chromatography Aqueous solution limit of detection concentration (parameters) quantitative analysis Chemistry Organic Chemistry Water Volatile fatty acids (VFAs) volatile fatty acids anaerobic digestion chromatography fatty acid analysis Química General Medicine Fatty Acids Volatile validation study Gas chromatography Direct injection Quantification methods aqueous solution volatile fatty acid |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Biblos-e Archivo. Repositorio Institucional de la UAM |
| DOI: | 10.1016/j.chroma.2015.08.008 |
| Popis: | 31 Páginas; 19 Tablas The performance parameters of volatile fatty acids (VFAs) measurements were assessed for the first time by a multi-laboratory validation study among 13 laboratories. Two chromatographic techniques (GC and HPLC) and two quantification methods such as external and internal standard (ESTD/ISTD) were combined in three different methodologies GC/ESTD, HPLC/ESTD and GC/ISTD. Linearity evaluation of the calibration functions in a wide concentration range (10–1000 mg/L) was carried out using different statistical parameters for the goodness of fit. Both chromatographic techniques were considered similarly accurate. The use of GC/ISTD, despite showing similar analytical performance to the other methodologies, can be considered useful for the harmonization of VFAs analytical methodology taking into account the normalization of slope values used for the calculation of VFAs concentrations. Acceptance criteria for VFAs performance parameters of the multi-laboratory validation study should be established as follows: (1) instrument precision (RSDINST ≤ 1.5%); (2) linearity (R2 ≥ 0.998; RSDSENSITIVITY ≤ 4%; REMAX ≤ 8%; REAVER ≤ 3%); (3) precision (RSD ≤ 1.5%); (4) trueness (recovery of 97–103%); (5) LOD (≤3 mg/L); and (6) LOQ (10 mg/L). The participating research laboratories are acknowledged for their interest to harmonize the analytical performance of VFAs measurements, including environmental biotechnology laboratory (LBE) of EPFL – Environmental Engineering Institute (Lausanne, Switzerland) managed by Dr. Christof Holliger. In addition, all the individual analysts who participated with carrying out the chromatographic analyses are recognized as very important contributors for this interlaboratory work. Without their contribution and excellent work none of the results reported could have been obtained. |
| Databáze: | OpenAIRE |
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