Dynamics of the full length and mutated heat shock factor 1 in human cells
| Autor: | Eric G. Folco, Edwige Col, Gaetan Herbomel, Claire Vourc'h, Antoine Delon, Yves Usson, Meike Kloster-Landsberg, Catherine Souchier |
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| Přispěvatelé: | INSERM U823, équipe 10 (Stress et Dynamique de l'Organisation du Génome), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), MOTIV, Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Dynamique Cellulaire et Tissulaire- Interdisciplinarité, Modèles & Microscopies (TIMC-IMAG-DyCTiM), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Région Rhône-Alpes, CIBLE 2009, Association Recherche contre le cancer, Souchier, Catherine, Matériaux, Optique et Techniques Instrumentales pour le Vivant (MOTIV-LIPhy), Dynamiques Cellulaire, Tissulaire & Microscopie fonctionnelle (TIMC-IMAG-DyCTiM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Macromolecular Assemblies
Cell Membrane Permeability Intracellular Space lcsh:Medicine RNA polymerase II Chemical Fractionation Biochemistry Diffusion Mice molecular diffusion 0302 clinical medicine Molecular cell biology Heat Shock Transcription Factors Nucleic Acids Fluorescence Correlation Spectroscopy HSF1 lcsh:Science transcription factor Image Cytometry Cellular Stress Responses 0303 health sciences Multidisciplinary [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Physics Cellular Structures Chromatin Cell biology DNA-Binding Proteins Protein Transport Eukaryotic Cells Gene Knockdown Techniques MESH: enhanced green fluorescent protein Interdisciplinary Physics Heat shock factor Cellular Types living cells Research Article Fluorescence Recovery After Photobleaching Protein Binding Subcellular Fractions Transcriptional Activation [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] DNA transcription Green Fluorescent Proteins Biophysics MESH: heat shock transcription factor [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology 03 medical and health sciences Stress Physiological Animals Humans HSP70 Heat-Shock Proteins protein interaction Heat shock DNA binding Transcription factor [SDV.BC] Life Sciences [q-bio]/Cellular Biology 030304 developmental biology Cell Nucleus lcsh:R fungi Fluorescence recovery after photobleaching Proteins DNA-binding domain DNA Cell Nucleus Structures molecular dynamics Protein Structure Tertiary Molecular Weight Spectrometry Fluorescence Mutation biology.protein FRAP RNA Mutant Proteins lcsh:Q Gene expression 030217 neurology & neurosurgery Cytometry Heat-Shock Response MESH: Fluorescence Recovery After Photobleaching MESH: DNA-Binding Proteins HeLa Cells Transcription Factors |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (7), pp.e67566. ⟨10.1371/journal.pone.0067566⟩ PLoS ONE, 2013, 8 (7), pp.e67566. ⟨10.1371/journal.pone.0067566⟩ PLoS ONE, Vol 8, Iss 7, p e67566 (2013) |
| ISSN: | 1932-6203 |
| Popis: | International audience; Heat shock factor 1 is the key transcription factor of the heat shock response. Its function is to protect the cell against the deleterious effects of stress. Upon stress, HSF1 binds to and transcribes hsp genes and repeated satellite III (sat III) sequences present at the 9q12 locus. HSF1 binding to pericentric sat III sequences forms structures known as nuclear stress bodies (nSBs). nSBs represent a natural amplification of RNA pol II dependent transcription sites. Dynamics of HSF1 and of deletion mutants were studied in living cells using multi-confocal Fluorescence Correlation Spectroscopy (mFCS) and Fluorescence Recovery After Photobleaching (FRAP). In this paper, we show that HSF1 dynamics modifications upon heat shock result from both formation of high molecular weight complexes and increased HSF1 interactions with chromatin. These interactions involve both DNA binding with Heat Shock Element (HSE) and sat III sequences and a more transient sequence-independent binding likely corresponding to a search for more specific targets. We find that the trimerization domain is required for low affinity interactions with chromatin while the DNA binding domain is required for site-specific interactions of HSF1 with DNA. |
| Databáze: | OpenAIRE |
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