Treatment of Graves' disease with methimazole in children alters the proliferation of Treg cells and CD3+ T lymphocytes
| Autor: | Ewelina Grywalska, Jacek Roliński, Maria Klatka, Jerzy Foerster, Marta Cyman, Magdalena Wasiak, Lucyna Kaszubowska, Leszek Szewczyk |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Histology
Adolescent CD3 Complex CD3 T cell Graves' disease T-Lymphocytes Lymphocyte proliferation T-Lymphocytes Regulatory Pathology and Forensic Medicine Interleukin 21 Antithyroid Agents Medicine Humans Lymphocyte Count Child Cells Cultured Cell Proliferation Autoimmune disease Methimazole biology business.industry Cell growth General Medicine T lymphocyte medicine.disease Graves Disease medicine.anatomical_structure Case-Control Studies Immunology biology.protein Female business |
| Zdroj: | Folia histochemica et cytobiologica. 52(1) |
| ISSN: | 1897-5631 |
| Popis: | Almost all cases of hyperthyroidism in children result from Graves' disease (GD). Recent studies have confirmed a significant role of T regulatory cells (Tregs) in the development of autoimmune diseases. However, the interactions between T cell responses and Treg proliferation in GD are still poorly understood. The aim of this study was to assess the proliferation of Treg cells (Tregs) and CD3+ T lymphocytes isolated from 50 children with GD before and after treatment with the thyreostatic drug methimazole (MMI). The proliferation rates, measured by methyl-3H-thymidyne incorporation, of CD3+ cells and Tregs stimulated with mitogen phorbol 12-myristate 13-acetate (PMA) were compared with those of unstimulated cells. The proliferation rates of both PMA-stimulated and unstimulated CD3+ cells prior to treatment with MMI were significantly higher than after treatment. Simultaneously, the proliferation rates of both PMA-stimulated and unstimulated Tregs were significantly lower before MMI treatment. Moreover, we observed higher cell proliferation rates of unstimulated and PMA-stimulated Tregs before the initiation of MMI therapy and after treatment in patients who had no relapse of hyperthyroidism. There was a positive correlation between the CD3+ cells proliferation rate before MMI treatment and fT3, as well as fT4 concentration in peripheral blood. The proliferation rates of CD3+ T cells before and after MMI treatment positively correlated with the TSI index. Thus, children suffering from Graves' disease presented lower Tregs proliferative potential compared with CD3+ T cells. Cocultures of CD3+ T cells and Tregs showed that Tregs were not capable of efficiently inhibiting the proliferation of CD3+ T cells in GD patients. Conclusions. MMI treatment reduced the proliferative activity of CD3+ T cells in pediatric GD patients and increased the proliferation rate of Tregs. We suggest that Treg cells that are partly dysfunctional in GD disease are probably suppressed by CD3+ T cells and that methimazole exerts some immunomodulatory effects. |
| Databáze: | OpenAIRE |
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