Molecular epidemiology of HIV in two highly endemic areas of northeastern South Africa
| Autor: | Marie-Louise Hammarskjold, Tracy Masebe, David Rekosh, Benson Chuks Iweriebor, Jeffrey Mphahlele, Lufuno Mavhandu, Pascal O. Bessong |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Genotype medicine.medical_treatment viruses Population Molecular Sequence Data Mutation Missense HIV Infections Drug resistance Biology Article South Africa Viral Proteins Virology Genetic variation medicine Prevalence Cluster Analysis Humans education Child Phylogeny Genetics education.field_of_study Genetic diversity Molecular Epidemiology Protease Molecular epidemiology virus diseases Genetic Variation General Medicine Sequence Analysis DNA Middle Aged Reverse transcriptase Amino Acid Substitution HIV-1 RNA Viral Female |
| Zdroj: | Archives of virology. 157(3) |
| ISSN: | 1432-8798 |
| Popis: | There is paucity of data on the genetic landscape of HIV-1 viruses circulating in the Limpopo Province of northeastern South Africa. Here, we examine the genetic diversity of viruses from Bela-Bela and Musina, two towns with high HIV prevalence. Between June 2007 and March 2008, blood samples were collected from antiretroviral-drug-naive individuals. Viruses were analyzed for genetic subtypes and drug resistance mutations. All of the viruses in these samples were shown by phylogenetic analysis based on gag p17, gag p24, reverse transcriptase, protease and envelope C2-C3 gene regions to belong to HIV-1 subtype C. Two of 44 reverse transcriptase sequences (4.5%) contained N rather than the consensus K at position 103. The K103N mutation is normally associated with resistance to NNRTIs. No major mutations were observed in the protease gene. However, several polymorphisms and amino acid changes normally considered to be minor drug resistance mutations were observed in the protease sequences. These results suggest that HIV-1 subtype C remains the predominant variant responsible for the epidemic in northeastern South Africa and that the prevalence of drug-resistant viruses among the naive population is low. |
| Databáze: | OpenAIRE |
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