Comparative Plasma Lipidome between Human and Cynomolgus Monkey: Are Plasma Polar Lipids Good Biomarkers for Diabetic Monkeys?
| Autor: | Wei Fun Cheong, Sin Man Lam, Markus R. Wenk, Francine Marie Gregoire, Guanghou Shui, Jeffrey William Stebbins, Jun Kusonoki, Buu Duyen Lam |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Ceramide
Anatomy and Physiology Plasmalogen Clinical Research Design Plasmalogens lcsh:Medicine Endocrine System Biology Biochemistry chemistry.chemical_compound Model Organisms Endocrinology Blood plasma Diabetes Mellitus Animals Humans Animal Models of Disease lcsh:Science Phosphatidylethanolamine Liquid Chromatography Diabetic Endocrinology Sphingolipids Chromatography Multidisciplinary lcsh:R Phosphatidylglycerols Phosphatidic acid Animal Models Fasting Feeding Behavior Lipidome Lipid Metabolism Sphingolipid Lipids Chemistry Macaca fascicularis Metabolism chemistry Blood Chemistry Medicine lcsh:Q lipids (amino acids peptides and proteins) Sphingomyelin Macaque Biomarkers Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 5, p e19731 (2011) |
| ISSN: | 1932-6203 |
| Popis: | Background Non-human primates (NHP) are now being considered as models for investigating human metabolic diseases including diabetes. Analyses of cholesterol and triglycerides in plasma derived from NHPs can easily be achieved using methods employed in humans. Information pertaining to other lipid species in monkey plasma, however, is lacking and requires comprehensive experimental analysis. Methodologies/Principal Findings We examined the plasma lipidome from 16 cynomolgus monkey, Macaca fascicularis, using liquid chromatography coupled with mass spectrometry (LC/MS). We established novel analytical approaches, which are based on a simple gradient elution, to quantify polar lipids in plasma including (i) glycerophospholipids (phosphatidylcholine, PC; phosphatidylethanolamine, PE; phosphatidylinositol, PI; phosphatidylglycerol, PG; phosphatidylserine, PS; phosphatidic acid, PA); (ii) sphingolipids (sphingomyelin, SM; ceramide, Cer; Glucocyl-ceramide, GluCer; ganglioside mannoside 3, GM3). Lipidomic analysis had revealed that the plasma of human and cynomolgus monkey were of similar compositions, with PC, SM, PE, LPC and PI constituting the major polar lipid species present. Human plasma contained significantly higher levels of plasmalogen PE species (p |
| Databáze: | OpenAIRE |
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