Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts

Autor:	Van den Bossche, Lindsay, Schoonenberg, Vivien A C, Burgener, Iwan A, Penning, Louis C, Schrall, Ingrid M, Kruitwagen, Hedwig S, van Wolferen, Monique E, Grinwis, Guy C M, Kummeling, Anne, Rothuizen, Jan, van Velzen, Jeroen F., Stathonikos, Nikolas, Molenaar, Martijn R, Helms, Bernd J, Brouwers, Jos F H M, Spee, Bart, van Steenbeek, Frank G, dCSCA RMSC-1, dCSCA AVR, dPB CR, Veterinair Pathologisch Diagnostisch Cnt, PB AVM, dB&C FR-RMSC FR
Přispěvatelé:	dCSCA RMSC-1, dCSCA AVR, dPB CR, Veterinair Pathologisch Diagnostisch Cnt, PB AVM, dB&C FR-RMSC FR
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	0301 basic medicine Steatosis lcsh:Medicine Pathology and Laboratory Medicine Biochemistry Gastroenterology Mass Spectrometry Cytopathology Pathogenesis chemistry.chemical_compound Non-alcoholic Fatty Liver Disease Medicine and Health Sciences Portasystemic Shunt Surgical Organ Cultures lcsh:Science Chromatography High Pressure Liquid Phospholipids Mammals Multidisciplinary Agricultural and Biological Sciences(all) Liver Diseases Fatty Acids Fatty liver Eukaryota Lipids Organoids Liver Vertebrates Biological Cultures Neutral Lipids Shunt (electrical) Research Article medicine.medical_specialty Gastroenterology and Hepatology Research and Analysis Methods 03 medical and health sciences Dogs Internal medicine medicine Organoid Animals Triglyceride Biochemistry Genetics and Molecular Biology(all) business.industry lcsh:R Organisms Biology and Life Sciences Lipid metabolism Metabolism Lipid Metabolism medicine.disease Fatty Liver 030104 developmental biology chemistry Anatomical Pathology Amniotes lcsh:Q business Genetics and Molecular Biology(all)
Zdroj:	PLoS ONE, Vol 12, Iss 10, p e0186491 (2017) PLoS ONE, 12(10). Public Library of Science PLoS One, 12(10). Public Library of Science PLoS ONE
ISSN:	1932-6203
Popis:	Non-alcoholic fatty liver disease (NAFLD) is a poorly understood multifactorial pandemic disorder. One of the hallmarks of NAFLD, hepatic steatosis, is a common feature in canine congenital portosystemic shunts. The aim of this study was to gain detailed insight into the pathogenesis of steatosis in this large animal model. Hepatic lipid accumulation, gene-expression analysis and HPLC-MS of neutral lipids and phospholipids in extrahepatic (EHPSS) and intrahepatic portosystemic shunts (IHPSS) was compared to healthy control dogs. Liver organoids of diseased dogs and healthy control dogs were incubated with palmitic- and oleic-acid, and lipid accumulation was quantified using LD540. In histological slides of shunt livers, a 12-fold increase of lipid content was detected compared to the control dogs (EHPSS P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09ca1b9fd78a468a18eaaa8f0895d71c http://europepmc.org/articles/PMC5648188?pdf=render Zobrazit plný text záznamu Plný text ve formátu PDF