Ex vivo/in vitro protective effect of myricetin bulk and nano-forms on PhIP-induced DNA damage in lymphocytes from healthy individuals and pre-cancerous MGUS patients

Autor: Mohammad Isreb, Shabana Akhtar, Lisa Newton, Rajendran C. Gopalan, Mojgan Najafzadeh, Diana Anderson
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Health
Toxicology and Mutagenesis
Binucleated cells
Mutagen
Ataxia Telangiectasia Mutated Proteins
Toxicology
medicine.disease_cause
Monoclonal Gammopathy of Undetermined Significance
Antioxidants
chemistry.chemical_compound
0302 clinical medicine
Lymphocytes
Nanotoxicology
Cells
Cultured
Aged
80 and over
Micronucleus Tests
Myricetin
Imidazoles
Antimutagenic Agents
General Medicine
Middle Aged
030220 oncology & carcinogenesis
Micronucleus test
Female
Comet Assay
Signal Transduction
PhIP
Adult
Bulk and nano-forms
DNA damage
Young Adult
03 medical and health sciences
medicine
Humans
Micronuclei
Chromosome-Defective
Aged
Flavonoids
Pre-cancerous patients
P53
Molecular biology
Comet assay
Oxidative Stress
ATR
030104 developmental biology
chemistry
Case-Control Studies
Nanoparticles
Tumor Suppressor Protein p53
Ex vivo
Oxidative stress
DNA Damage
Mutagens
Zdroj: Archives of Toxicology
ISSN: 1432-0738
0340-5761
DOI: 10.1007/s00204-020-02754-x
Popis: 2-Amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) is a central dietary mutagen, produced when proteinaceous food is heated at very high temperatures potentially causing DNA strand breaks. This study investigates the protective potential of a well-researched flavonoid, myricetin in its bulk and nano-forms against oxidative stress induced ex vivo/in vitro by PhIP in lymphocytes from pre-cancerous monoclonal gammopathy of undetermined significance (MGUS) patients and those from healthy individuals. The results from the Comet assay revealed that in the presence of myricetin bulk (10 µM) and myricetin nano (20 µM), the DNA damage caused by a high dose of PhIP (100 µM) was significantly (P P P
Databáze: OpenAIRE
Externí odkaz: