Effects of an oral hydro-ethanolic purple coneflower extract on performance, clinical health and immune parameters in calves
| Autor: | Raffael Fricker, Andrea Vögtlin, Anna Bieber, Hannah Ayrle, Meike Mevissen, Michael Walkenhorst, Martin Kaske, Olga Wellnitz, Rupert M. Bruckmaier |
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| Rok vydání: | 2021 |
| Předmět: |
Male
040301 veterinary sciences Bovine respiratory disease Health and welfare Hematocrit Echinacea 0403 veterinary science Blood cell Andrology 03 medical and health sciences Double-Blind Method Oral administration medicine Animals Seroconversion 030304 developmental biology 0303 health sciences 630 Agriculture General Veterinary biology medicine.diagnostic_test Plant Extracts business.industry Vaccination Beef cattle Viral Vaccines 04 agricultural and veterinary sciences Eosinophil medicine.disease Red blood cell medicine.anatomical_structure biology.protein 570 Life sciences Cattle Female Antibody business Bluetongue virus |
| Zdroj: | Research in Veterinary Science. 138:148-160 |
| ISSN: | 0034-5288 |
| Popis: | The objective of this randomized, placebo-controlled, double-blinded field trial was to investigate the effects of oral administration of purple coneflower (Echinacea purpurea L. (EP)) on performance, health and immune parameters in calves. Calves (n = 27) were enrolled to three groups (9 calves per group): 0.5 g EP/calf per day (ECL), 5 g EP/calf per day (ECH) or placebo. Calves were vaccinated with Bluetongue-Virus (BTV) serotype 4 vaccine to investigate EPs effects on seroconversion. Clinical and performance parameters, inter alia body weight, health and milk intake were recorded for 57 days. Blood samples were analyzed for BTV antibodies and IgG by ELISA, white and red blood cell counts by flow cytometry and mRNA abundance of various inflammatory markers in leukocytes (IL-1β, IL-8, tumor necrosis factor α (TNFα), cyclooxygenase 2 (Cox-2) and prostaglandin E synthase) was studied. The findings demonstrated no differences between groups regarding performance parameters. In all groups, calves suffered from diarrhea for a minimum of 2 days, but EP reduced the number of diarrhea days by 44% in ECL and increased the body temperature. Interestingly, ECL resulted in an increased number of respiratory disease days during the follow-up period. EP did not change blood cell and IgG counts, whereas eosinophil granulocytes were reduced in ECL. Decreased levels of hemoglobin and hematocrit were found in ECH. Prostaglandin E synthase levels in leukocytes were higher in ECL and ECH, whereas no differences were obtained for IL-1β, IL-8, TNFα and Cox-2. Due to the unexpected occurrence of BTV seropositive calves before the first vaccination, 13 calves were excluded from the evaluation on seroconversion and no statistical analyses could be performed regarding antibody production. BTV-4 antibodies were not produced in 4 placebo-calves, whereas 4 of 5 and 1 of 6 ECL- and ECH-calves produced antibodies. Further investigations are needed to draw final conclusions on mode of action and efficacy of EP in calves. |
| Databáze: | OpenAIRE |
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