Peptide synthesis catalyzed by papain at alkaline pH values
| Autor: | Yu. V. Mitin, E. Yu. Gorbunova, N.P. Zapevalova |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Peptide Hydrogen-Ion Concentration Biochemistry Amino acid Kinetics Structure-Activity Relationship Hydrolysis chemistry.chemical_compound Papain chemistry Yield (chemistry) Reagent Solvents Peptide synthesis Organic chemistry Indicators and Reagents Oligopeptides Alkyl |
| Zdroj: | International Journal of Peptide and Protein Research. 23:528-534 |
| ISSN: | 0367-8377 |
| DOI: | 10.1111/j.1399-3011.1984.tb02754.x |
| Popis: | The synthesis of peptides in the presence of papain at pH 8-9.5 is described. Starting substances are acylamino acid alkyl esters (the carboxyl component) and amides or tert.-butylesters of amino acids, as well as peptide (the amino component). Under such conditions secondary hydrolysis is not essential, making the synthesis of peptides soluble in aqueous medium. The yield of peptides is 50-94%. The effect of different factors (temperature, solvents, reagent concentrations) on the result of the reaction has been studied. It has been found that an excess of the carboxyl component is expedient to increase the yield of peptides. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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