Single Nucleotide rs760370 Polymorphism at the Main Ribavirin Transporter Gene Detection by PCR-RFLP Assay Compared with the TaqMan Assay and Its Relation to Sustained Virological Response in Chronic HCV Patients Treated with Pegylated Interferon-Ribavirin Therapy
| Autor: | Rabab Fouad, Wafaa Ezzat, Ahmed Heiba, Marwa Sheta, Mervat M. Khorshied, Khaled S. Zachariah, Marwa Khairy |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Genotype Immunology Single-nucleotide polymorphism Hepacivirus Real-Time Polymerase Chain Reaction Polymerase Chain Reaction Polymorphism Single Nucleotide Virus Equilibrative Nucleoside Transporter 1 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pegylated interferon Virology Ribavirin TaqMan Medicine SNP Humans Alleles business.industry virus diseases Interferon-alpha Cell Biology Hepatitis C Chronic Middle Aged digestive system diseases Regimen 030104 developmental biology Treatment Outcome chemistry 030211 gastroenterology & hepatology Drug Therapy Combination Female Restriction fragment length polymorphism business Polymorphism Restriction Fragment Length medicine.drug |
| Zdroj: | Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 37(2) |
| ISSN: | 1557-7465 |
| Popis: | Ribavirin clearly plays a role in chronic hepatitis C treatment response. The equilibrative nucleoside transporter-1 codified by SLC29A1 gene has been associated with ribavirin uptake into hepatocytes and erythrocytes. rs760370AG single nucleotide polymorphism (SNP) at the SLC29A1 gene may have a role in ribavirin-based regimen treatment response. Accuracy of the polymerase-chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay compared with the TaqMan assay for the detection of the SNP rs760370 at the main ribavirin transporter gene and its relation to sustained virological response in chronic hepatitis C virus (HCV) patients treated with pegylated interferon-ribavirin therapy. The study included 100 chronic HCV patients who were treated with pegylated interferon-ribavirin therapy. The patients were categorized according to the treatment response into responders (50 patients) and null responders (50 patients). rs760370 SNP was measured using TaqMan 5-nuclease assay and by the newly developed PCR-based RFLP assay. The overall accuracy of the newly developed PCR-RFLP assay compared with the TaqMan assay for rs760370 polymorphism detection was 100%. Allelic frequencies at rs760370 were as follows: A/A genotype (28%), A/G genotype (58%), and G/G genotype (14%). Treatment response was not significantly related with rs760370 polymorphism (P = 0.5). Ribavirin-induced anemia was good predictor of sustained virological response (P = 0.001), but was not related to rs760370 polymorphism (P = 0.92). PCR-RFLP assay is an accurate, cost-effective method in the detection of rs760370 compared with TaqMan assay. rs760370 SNP cannot serve as predictor of response in chronic HCV patients treated with interferon ribavirin therapy. |
| Databáze: | OpenAIRE |
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