Cutting Edge: Memory B Cell Survival and Function in the Absence of Secreted Antibody and Immune Complexes on Follicular Dendritic Cells
| Autor: | Shannon M. Anderson, Mark J. Shlomchik, Lynn G. Hannum |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cell Survival
Genes Immunoglobulin Heavy Chain Immunology Population Mice Transgenic Antigen-Antibody Complex Mice Immune system medicine Animals Immunology and Allergy Memory B cell education B cell B-Lymphocytes education.field_of_study biology Follicular dendritic cells Germinal center Germinal Center Cell biology B-1 cell medicine.anatomical_structure Antibody Formation biology.protein Immunization Antibody Immunologic Memory Dendritic Cells Follicular |
| Zdroj: | The Journal of Immunology. 176:4515-4519 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.176.8.4515 |
| Popis: | Ag, in the form of immune complexes retained on follicular dendritic cells, has been implicated in the development and maintenance of B cell memory. We addressed this question using a H chain transgenic (Tg) mouse model that lacks secreted Ig (mIg), and thus does not deposit Ag-containing immune complexes. We compared the ability of the mIg strain and a control Tg strain, which secretes IgM, to develop and maintain long-lived memory cells. After immunization, there was an increase of Ag-specific B cells in both strains that was maintained for at least 20 wk. We labeled the long-lived Ag-specific cells with BrdU and found that this population was similarly maintained. In addition, both Tgs were able to maintain a functional memory response as measured by secondary germinal center reactions. Our studies indicate that localization of Ag on follicular dendritic cells is not necessary for development and maintenance of B cell memory. |
| Databáze: | OpenAIRE |
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