The effect of isosorbide dinitrate on uterine and ovarian blood flow in cycling and early pregnant mares: A pilot study

Autor: Johannes Lüttgenau, D. Zoller, S. Steffen, Heinrich Bollwein
Přispěvatelé: University of Zurich, Zoller, D
Rok vydání: 2016
Předmět:
Vasodilator Agents
animal diseases
Physiology
Pilot Projects
Isosorbide Dinitrate
Ovarian artery
0302 clinical medicine
Food Animals
Pregnancy
Small Animals
Early pregnancy
reproductive and urinary physiology
media_common
030219 obstetrics & reproductive medicine
630 Agriculture
Estrous cycle
04 agricultural and veterinary sciences
Female
Perfusion
Blood Flow Velocity
3403 Food Animals
3402 Equine
medicine.drug
Infertility
endocrine system
medicine.medical_specialty
media_common.quotation_subject
Placebo
03 medical and health sciences
medicine.artery
medicine
Animals
Horses
Ovulation
Genital blood flow
Gynecology
urogenital system
Equine
business.industry
Ovary
Uterus
Hemodynamics
0402 animal and dairy science
Nitric oxide
medicine.disease
040201 dairy & animal science
10187 Department of Farm Animals
3404 Small Animals
570 Life sciences
biology
Animal Science and Zoology
sense organs
1103 Animal Science and Zoology
Isosorbide dinitrate
business
Zdroj: Theriogenology. 85:1562-1567
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2016.01.009
Popis: Poor uterine perfusion has been proposed as a cause of infertility in mares. The objective of this study was to investigate the effect of isosorbide dinitrate (ISDN), a nitric oxide donor, on uterine and ovarian blood flow resistance during diestrus and early pregnancy in mares. Six Trotter mares, aged 7 to 14 years, were examined daily during the first 11 days of three diestrous periods, and five of those mares were also examined during the first 11 days of two pregnancies. Six mares randomly received a placebo, a low dose (30 mg, ISDN30), or a high dose of ISDN (60 mg, ISDN60) through three nonconsecutive cycles. The treatments were administered orally, every 12 hours from Day 1 to 11 of the cycle (Day 0 = ovulation). Five of the 6 mares received a placebo or 60 mg of ISDN orally every 12 hours from Day 1 to 11 of pregnancy. The mares were short cycled on Day 12 of each trial. Transrectal color Doppler was used to determine blood flow resistance semiquantitatively and expressed as pulsatility index. Mean pulsatility index of both uterine arteries combined and of the dominant (ipsilateral to the CL) ovarian artery was lower (treatment effects: P ≤ 0.01; time effects: P ≤ 0.002) in mares receiving 30 mg or 60 mg of ISDN compared with placebo-treated mares. Blood flow resistance in the dominant ovarian artery was lower in ISDN-treated pregnant mares than in placebo-treated pregnant and cycling mares (treatment effect: P = 0.04; time effect: P = 0.003). Isosorbide dinitrate increases uterine and ovarian perfusion in cycling mares and ovarian perfusion in early pregnant mares. Further studies are needed to investigate these effects in relation to fertility of the mare.
Databáze: OpenAIRE
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