Vaginal heat shock protein expression in symptom-free women with a history of recurrent vulvovaginitis
| Autor: | José Antonio Simões, A. Neuer, Steven S. Witkin, Paulo César Giraldo, Ayrton Ribeiro-Filho, Irina Korneeva |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Chlamydia trachomatis Mycoplasma hominis medicine.disease_cause Polymerase Chain Reaction Vaginal disease Recurrence Heat shock protein Candida albicans medicine Humans HSP70 Heat-Shock Proteins Papillomaviridae Vaginitis biology business.industry Interleukins Obstetrics and Gynecology Chaperonin 60 Middle Aged Vulvovaginitis biology.organism_classification medicine.disease medicine.anatomical_structure Gene Expression Regulation Case-Control Studies Vagina Immunology Female Bacterial vaginosis business Ureaplasma urealyticum |
| Zdroj: | American Journal of Obstetrics and Gynecology. 180:524-529 |
| ISSN: | 0002-9378 |
| Popis: | Objectives: The cause of recurrent vulvovaginitis remains unexplained in most cases. Heat shock protein synthesis is induced under conditions of stress; its presence in vaginal samples from women who were between episodes of recurrent vulvovaginitis thus might reflect a persistent perturbation in the local milieu. Study Design: We undertook an evaluation by means of enzyme-linked immunosorbent assay of 60-kd heat shock protein and inducible 70-kd heat shock protein expressions in vaginal wash samples from 24 symptom-free women with a history of recurrent vulvovaginitis and 19 matched control subjects. The samples were also tested for Candida albicans , Chlamydia trachomatis , Ureaplasma urealyticum , Mycoplasma hominis, and human papillomavirus by polymerase chain reaction; for bacterial vaginosis by clinical and microbiologic evaluation; and for interleukin 10, interleukin 1, interleukin 8, RANTES, and eotaxin by enzyme-linked immunosorbent assay. Results: The presence of 60-kd heat shock protein was detected in 11 women with recurrent vulvovaginitis (45.8%) and 1 control subject (5.3%, P = .005). Similarly, 70-kd heat shock protein was present in 8 patients with recurrent vulvovaginitis (33.3%) and no control subjects ( P = .005). The presence of 60-kd heat shock protein and the presence of 70-kd heat shock protein were correlated with each other ( P = .02), as were both 60-kd heat shock protein ( P = .006) and 70-kd heat shock protein ( P = .01) correlated with IL-10. There was no relation between the presence of 60-kd heat shock protein or 70-kd heat shock protein and detection of IL-1, IL-8, or any microorganism. Conclusion: The expression of heat shock proteins and IL-10 in the vaginas of women with a history of recurrent vulvovaginitis but not in the vaginas of control subjects suggests the existence of differences in the vaginal milieu between the 2 groups, even when both are without vaginal symptoms. (Am J Obstet Gynecol 1999;180:524-9.) |
| Databáze: | OpenAIRE |
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