Electron tomography—a tool for ultrastructural 3D visualization in cell biology and histology
| Autor: | Josef Neumüller |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Electron Microscope Tomography Cell biology Histology Cytological Techniques Pharmacology toxicology Biology 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine Computer graphics (images) Ultrastruktur Humans Medicine Zellbiologie 3D-Darstellung Histologie Geriatrics gerontology business.industry Main Topic General Medicine Elektronentomografie Visualization 030104 developmental biology Electron tomography 3D visualization Ultrastructure business 030217 neurology & neurosurgery |
| Zdroj: | Wiener Medizinische Wochenschrift (1946) |
| ISSN: | 1563-258X 0043-5341 |
| DOI: | 10.1007/s10354-018-0646-y |
| Popis: | Summary Electron tomography (ET) was developed to overcome some of the problems associated reconstructing three-dimensional (3D) images from 2D election microscopy data from ultrathin slices. Virtual sections of semithin sample are obtained by incremental rotation of the target and this information is used to assemble a 3D image. Herein, we provide an instruction to ET including the physical principle, possibilities, and limitations. We review the development of innovative methods and highlight important investigations performed in our department and with our collaborators. ET has opened up the third dimension at the ultrastructural level and represents a milestone in structural molecular biology. |
| Databáze: | OpenAIRE |
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