Mechanisms of Spica Prunellae against thyroid-associated Ophthalmopathy based on network pharmacology and molecular docking
| Autor: | Xianzhi Li, Yuhan Zhang, Lin Liao, Congcong Guo, Jianjun Dong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
AKT1 Computational biology Biology Molecular Docking Simulation 03 medical and health sciences 0302 clinical medicine Network pharmacology Effective treatment Humans Protein Interaction Maps Prunella Thyroid-associated ophthalmopathy Thyroid-Associated Ophthalmopathy Virtual screening Mechanism (biology) lcsh:Other systems of medicine lcsh:RZ201-999 Graves Ophthalmopathy 030104 developmental biology Complementary and alternative medicine Spica Prunellae 030220 oncology & carcinogenesis Molecular docking Signal transduction TAO Drugs Chinese Herbal Research Article |
| Zdroj: | BMC Complementary Medicine and Therapies BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-16 (2020) |
| ISSN: | 2662-7671 |
| Popis: | Background Thyroid-associated ophthalmopathy (TAO) is an autoimmune inflammatory disorder, which lacks effective treatment currently. Spica Prunellae (SP) is popularly used for its anti-inflammatory and immune-regulating properties, indicating SP may have potential therapeutic value in TAO. Therefore, the purpose of this study is to identify the efficiency and potential mechanism of SP in treating TAO. Methods A network pharmacology integrated molecular docking strategy was used to predict the underlying molecular mechanism of treating TAO. Firstly, the active compounds of SP were obtained from TCMSP database and literature research. Then we collected the putative targets of SP and TAO based on multi-sources databases to generate networks. Network topology analysis, GO and KEGG pathway enrichment analysis were performed to screen the key targets and mechanism. Furthermore, molecular docking simulation provided an assessment tool for verifying drug and target binding. Results Our results showed that 8 targets (PTGS2, MAPK3, AKT1, TNF, MAPK1, CASP3, IL6, MMP9) were recognized as key therapeutic targets with excellent binding affinity after network analysis and molecular docking-based virtual screening. The results of enrichment analysis suggested that the underlying mechanism was mainly focused on the biological processes and pathways associated with immune inflammation, proliferation, and apoptosis. Notably, the key pathway was considered as the PI3K-AKT signaling pathway. Conclusion In summary, the present study elucidates that SP may suppress inflammation and proliferation and promote apoptosis through the PI3K-AKT pathway, which makes SP a potential treatment against TAO. And this study offers new reference points for future experimental research and provides a scientific basis for more widespread clinical application. |
| Databáze: | OpenAIRE |
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