Gas chromatography/atmospheric pressure chemical ionization/mass spectrometry for the analysis of organochlorine pesticides and polychlorinated biphenyls in human serum
| Autor: | Thanh Wang, Jessika Hagberg, Ingrid Ericson Jogsten, Dawei Geng, Rémi Rabasa-Lhoret, Bert van Bavel, Jérôme Ruzzin, Jody Dunstan |
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| Rok vydání: | 2016 |
| Předmět: |
Analytical chemistry
Atmospheric-pressure chemical ionization Signal-To-Noise Ratio 010501 environmental sciences Mass spectrometry 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry Tandem Mass Spectrometry Triple quadrupole mass spectrometry Hydrocarbons Chlorinated Physics::Atomic and Molecular Clusters Humans Physics::Atomic Physics Pesticides 0105 earth and related environmental sciences Chromatography Chemistry 010401 analytical chemistry Organic Chemistry Organochlorine pesticide General Medicine Polychlorinated Biphenyls Quantitative Biology::Genomics 0104 chemical sciences Atmospheric Pressure Gas chromatography |
| Zdroj: | Journal of Chromatography A. 1453:88-98 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2016.05.030 |
| Popis: | A method using a novel atmospheric pressure chemical ionization source for coupling gas chromatography (GC/APCI) to triple quadrupole mass spectrometry (MS/MS) for the determination of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) regulated by the Stockholm Convention is presented. One microliter injection of a six-point calibration curve of native PCBs and OCPs, ranging from 0.04 to 300pg/μL, was performed. The relative standard deviation (RSD) of the relative response factors (RRFs) was less than 15% with a coefficient of determination (r(2))0.995. Meanwhile, two calibration solutions (CS), CS 2 (0.4pg/μL) and CS 3 (4pg/μL) were analyzed to study the repeatability calculated for both area and RRFs. The RSD for RRF ranged from 3.1 to 16% and 3.6 to 5.5% for CS 2 and CS 3, respectively. The limits of detection (LOD) determined by peak-to-peak signal-to-noise ratio (S/N) of 3 were compared between the GC/APCI/MS/MS and a GC coupled to high resolution mass spectrometry (GC/HRMS) system. GC/APCI/MS/MS resulted in lower LOD for most of the compounds, except for PCB#74, cis-chlordane and trans-chlordane. GC/APCI/MS/MS and GC/HRMS were also compared by performing analysis on 75 human serum samples together with eight QA/QC serum samples. The comparison between GC/APCI/MS/MS system and GC/HRMS system for 16 of the targeted compounds was carried out. No statistically significant difference was discovered. Due to increased sensitivity and user friendly operation under atmospheric pressure, GC/APCI/MS/MS is a powerful alternative technique that can easily meet the specification of GC/HRMS. |
| Databáze: | OpenAIRE |
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