Effect of apigenin, kaempferol and resveratrol on the gene expression and protein secretion of tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) in RAW-264.7 macrophages
| Autor: | Monika Paul-Samojedny, Malgorzata Kowalczyk, Anna Fila-Danilow, Renata Suchanek-Raif, Paulina Borkowska, Marta Palacz-Wrobel, Jan Kowalski |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine medicine.medical_treatment Anti-Inflammatory Agents Gene Expression Pharmacology Resveratrol Cell Line Proinflammatory cytokine Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Stilbenes medicine Animals Secretion Apigenin Kaempferols Inflammation Tumor Necrosis Factor-alpha Macrophages food and beverages General Medicine Interleukin-10 Interleukin 10 RAW 264.7 Cells 030104 developmental biology Cytokine Biochemistry chemistry 030220 oncology & carcinogenesis Cytokines Tumor necrosis factor alpha Kaempferol |
| Zdroj: | Biomedicine & Pharmacotherapy. 93:1205-1212 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2017.07.054 |
| Popis: | Polyphenols such as apigenin, kaempferol or resveratrol are typically found in plants, including fruits, vegetables, herbs and spices, which have a wide range of biological functions such as antioxidative, anti-inflammatory, vasodilative, anticoagulative and proapoptotic. Discovering such multifunctional compounds in widely consumed plant-based products – ones that both inhibit the release of TNF-α from tissue macrophages and at the same time enhance the secretion of IL-10 – would be an important signpost in the quest for effective pharmacological treatment of numerous diseases that have an inflammatory etiology. The aim of the study is to investigate the impact of biologically active polyphenols such as apigenin, resveratrol and kaempferol on gene expression and protein secretion of IL-10 and TNF-α in line RAW-264.7. Cells were cultured under standard conditions. IL-10 and TNF-α genes expression were examined using QRT-PCR and to assess cytokines concentration ELISA have been used. Apigenin, kaempferol and resveratrol at a dose 30 μM significantly decrease the TNF-α expression and secretion. Apigenin decrease the IL-10 expression and secretion. Furthermore, increase in IL-10 secretion after administration of kaempferol and resveratrol were observed. In the process of administration of tested compounds before LPS, which activate macrophages, decrease of TNF-α secretion after apigenin and kaempferol and increase of IL-10 secretion after resveratrol were observed. The results of present work indicate that 1) apigenin, resveratrol and kaempferol may reduce the intensity of inflammatory processes by inhibiting the secretion of proinflammatory cytokine TNF-α, and resveratrol and kaempferol additionally by increasing the secretion of anti-inflammatory cytokine IL-10 2) the studies indicate the potentially beneficial – anti-inflammatory – impact of diet rich in products including apigenin, resveratrol and kaempferol. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect