Amylopectinosis in Fetal and Neonatal Quarter Horses
| Autor: | J. C. Fyfe, Margaret Z. Jones, F. A. Kennedy, J. A. Render, R. S. Common |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty 040301 veterinary sciences Amylopectin biology.animal_breed 0403 veterinary science Glycogen Storage Disease Type IV 03 medical and health sciences medicine Animals Horses Coloring Agents Muscle Skeletal Myopathy Inclusion Bodies Fetus General Veterinary biology Horse Congo Red 04 agricultural and veterinary sciences Anatomy Periodic Acid-Schiff Reaction Staining Basophilic Diastase Fetal Diseases Microscopy Electron 030104 developmental biology Animals Newborn Quarter horse Ultrastructure biology.protein Female Horse Diseases medicine.symptom Iodine |
| Zdroj: | Veterinary Pathology. 36:157-160 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1354/vp.36-2-157 |
| Popis: | Three Quarter Horses, a stillborn filly (horse No. 1), a female fetus aborted at approximately 6 months of gestation (horse No. 2), and a 1-month-old colt that had been weak at birth (horse No. 3), had myopathy characterized histologically by large spherical or ovoid inclusions in skeletal and cardiac myofibers. Smaller inclusions were also found in brain and spinal cord and in some cells of all other tissues examined. These inclusions were basophilic, red-purple after staining with periodic acid-Schiff (both before and after digestion with diastase), and moderately dark blue after staining with toluidine blue. The inclusions did not react when stained with Congo red. Staining with iodine ranged from pale blue to black. Their ultrastructural appearance varied from amorphous to somewhat filamentous. On the basis of staining characteristics and diastase resistance, we concluded that these inclusions contained amylopectin. A distinctly different kind of inclusion material was also present in skeletal muscle and tongue of horse Nos. 1 and 3. These inclusions were crystalline with a sharply defined ultrastructural periodicity. The crystals were eosinophilic and very dark blue when stained with toluidine blue but did not stain with iodine. Crystals sometimes occurred freely within the myofibers but more often were encased by deposits of amylopectin. This combination of histologic and ultrastructural features characterizes a previously unreported storage disease in fetal and neonatal Quarter Horses, with findings similar to those of glycogen storage disease type IV. We speculate that a severe inherited loss of glycogen brancher enzyme activity may be responsible for these findings. The relation of amylopectinosis to the death of the foals is unknown. |
| Databáze: | OpenAIRE |
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