Interfacing Digital Microfluidics with Ambient Mass Spectrometry Using SU-8 as Dielectric Layer
| Autor: | Tiina Sikanen, Gowtham Sathyanarayanan, Markus Haapala |
|---|---|
| Přispěvatelé: | Division of Pharmaceutical Chemistry and Technology, Drug Research Program, Preclinical Drug Formulation and Analysis group, Tiina Sikanen / Chemical Microsystems Lab |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
DESORPTION
Materials science digital microfluidics liquid-liquid extraction cytochrome P450 lcsh:Mechanical engineering and machinery Microfluidics 116 Chemical sciences Analytical chemistry 02 engineering and technology ambient mass spectrometry microreactor 01 natural sciences Article Liquid–liquid extraction Desorption ionization Sample preparation lcsh:TJ1-1570 Digital microfluidics Electrical and Electronic Engineering Desorption atmospheric pressure photoionization Detection limit CHIP Mechanical Engineering 010401 analytical chemistry desorption atmospheric pressure photoionization DROPLETS 021001 nanoscience & nanotechnology desorption/ionization drug metabolism 0104 chemical sciences SU-8 drug distribution Control and Systems Engineering 317 Pharmacy ELECTROWETTING-BASED ACTUATION Microreactor 221 Nano-technology 0210 nano-technology |
| Zdroj: | Micromachines Micromachines, Vol 9, Iss 12, p 649 (2018) Volume 9 Issue 12 |
| ISSN: | 2072-666X |
| Popis: | This work describes the interfacing of electrowetting-on-dielectric based digital microfluidic (DMF) sample preparation devices with ambient mass spectrometry (MS) via desorption atmospheric pressure photoionization (DAPPI). The DMF droplet manipulation technique was adopted to facilitate drug distribution and metabolism assays in droplet scale, while ambient mass spectrometry (MS) was exploited for the analysis of dried samples directly on the surface of the DMF device. Although ambient MS is well-established for bio- and forensic analyses directly on surfaces, its interfacing with DMF is scarce and requires careful optimization of the surface-sensitive processes, such as sample precipitation and the subsequent desorption/ionization. These technical challenges were addressed and resolved in this study by making use of the high mechanical, thermal, and chemical stability of SU-8. In our assay design, SU-8 served as the dielectric layer for DMF as well as the substrate material for DAPPI-MS. The feasibility of SU-8 based DMF devices for DAPPI-MS was demonstrated in the analysis of selected pharmaceuticals following on-chip liquid-liquid extraction or an enzymatic dealkylation reaction. The lower limits of detection were in the range of 1&ndash 10 pmol per droplet (0.25&ndash 1.0 µ g/mL) for all pharmaceuticals tested. |
| Databáze: | OpenAIRE |
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