In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers
| Autor: | Toon Brans, David Deruytter, Laszlo Vincze, Ine Van Nieuwenhove, Eva Vergucht, Michiel B. Vandegehuchte, Stephen Bauters, Jan Garrevoet, Manfred Burghammer, Filip Beunis, Colin R. Janssen, Maarten De Rijcke |
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| Přispěvatelé: | Universiteit Gent = Ghent University [Belgium] (UGENT), European Synchrotron Radiation Facility (ESRF) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Optical Tweezers Confocal [SDV]Life Sciences [q-bio] X-ray fluorescence Synchrotron radiation Nanotechnology single cell imaging Article law.invention law In vivo Microalgae biological model organisms Multidisciplinary optical tweezers microalgae Biology and Life Sciences Spectrometry X-Ray Emission Equipment Design Laser Fluorescence Beamline Optical tweezers Physics and Astronomy confocal Computer-Aided Design |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2015, 5, 8 p. ⟨10.1038/srep09049⟩ SCIENTIFIC REPORTS ESRF, 25th Users' meeting, Abstracts 'Scientific Reports ', vol: 5, pages: 9049-1-9049-8 (2015) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep09049⟩ |
| Popis: | We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in vivo elemental imaging in a two-dimensional (2D) projection mode in free-standing biological microorganisms or single cells, present in their aqueous environment. Using the model organism Scrippsiella trochoidea, a first proof of principle experiment at beamline ID13 of the European Synchrotron Radiation Facility (ESRF) demonstrates the feasibility of the OT XRF methodology, which is applied to study mixture toxicity of Cu-Ni and Cu-Zn as a result of elevated exposure. We expect that the new OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability. |
| Databáze: | OpenAIRE |
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