Behavior of fibrinogen fragments D and E in gel filtration experiments
| Autor: | David W. Towne, Elemer Mihalyi |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Gel electrophoresis
Chromatography Chemistry Elution Size-exclusion chromatography Hematology Fractionation Fibrinogen Human fibrinogen Fibrin Fibrinogen Degradation Products Heat coagulation Sephadex Spectrophotometry medicine Chromatography Gel Methods Animals Humans Cattle Electrophoresis Polyacrylamide Gel Trypsin Fibrinolysin Trypsin Inhibitors medicine.drug |
| Zdroj: | Thrombosis research. 8(1) |
| ISSN: | 0049-3848 |
| Popis: | Fibrinogen digests, at the completion of cleavage into the D and E fragments, were fractionated on a Sephadex G-200 column. Fragments D and E were estimated specifically across the main peak either by selective heat coagulation of fragment D, or by SDS-polyacrylamide gel electrophoresis. The resulting elution pattern of fragment E in the presence of fragment D was compared to that of isolated fragment E. With tryptic digests of bovine fibrinogen the patterns were identical, indicating no interaction of the two fragments. With plasmic digests of human fibrinogen, on the contrary, fragment E in the mixture eluted earlier, in an asymmetric boundary, extending across the whole main peak. This finding suggests some interaction between the two fragments. With tryptic digests of bovine fibrinogen fragment D can be obtained in a pure state by pooling the first half of the main peak of the Sephadex G-200 fractionation. The second half of the peak, after heat coagulation of fragment D, yields fragment E in a pure state. This method cannot be applied to plasmic digests of human fibrinogen because of the spreading of fragment E through the entire D peak. However, fragment E can be obtained in a pure state by heat coagulation of this peak. |
| Databáze: | OpenAIRE |
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