In situ hybridization analysis of the temporospatial expression of the midkine/pleiotrophin family in rat embryonic pituitary gland
Autor: | Takashi Yashiro, Dini Ramadhani, Ken Fujiwara, Takehiro Tsukada, Rita Maliza, Kotaro Horiguchi, Khongorzul Batchuluun, Alimuddin Tofrizal, Morio Azuma, Motoshi Kikuchi |
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Rok vydání: | 2014 |
Předmět: |
Pituitary gland
medicine.medical_specialty Cell signaling Histology Ectoderm In situ hybridization Pleiotrophin Pathology and Forensic Medicine Diencephalon Pregnancy Internal medicine medicine Animals Rats Wistar In Situ Hybridization Midkine biology Cell Biology Embryonic stem cell Rats Cell biology medicine.anatomical_structure Endocrinology Pituitary Gland biology.protein Cytokines Female Carrier Proteins |
Zdroj: | Cell and Tissue Research. 357:337-344 |
ISSN: | 1432-0878 0302-766X |
Popis: | Pituitary gland development is controlled by numerous signaling molecules, which are produced in the oral ectoderm and diencephalon. A newly described family of heparin-binding growth factors, namely midkine (MK)/pleiotrophin (PTN), is involved in regulating the growth and differentiation of many tissues and organs. Using in situ hybridization with digoxigenin-labeled cRNA probes, we detected cells expressing MK and PTN in the developing rat pituitary gland. At embryonic day 12.5 (E12.5), MK expression was localized in Rathke's pouch (derived from the oral ectoderm) and in the neurohypophyseal bud (derived from the diencephalon). From E12.5 to E19.5, MK mRNA was expressed in the developing neurohypophysis, and expression gradually decreased in the developing adenohypophysis. To characterize MK-expressing cells, we performed double-staining of MK mRNA and anterior pituitary hormones. At E19.5, no MK-expressing cells were stained with any hormone. In contrast, PTN was expressed only in the neurohypophysis primordium during all embryonic stages. In situ hybridization clearly showed that MK was expressed in primitive (immature/undifferentiated) adenohypophyseal cells and neurohypophyseal cells, whereas PTN was expressed only in neurohypophyseal cells. Thus, MK and PTN might play roles as signaling molecules during pituitary development. |
Databáze: | OpenAIRE |
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