Cytotoxic and genotoxic effects of abamectin, chlorfenapyr, and imidacloprid on CHOK1 cells
| Autor: | Alaa E. Bayoumi, Yasser Abobakr, Ali S. Al-Sarar, Hamdy I. Hussein |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cell Survival
Health Toxicology and Mutagenesis Glutathione reductase CHO Cells DNA Fragmentation Biology medicine.disease_cause Neonicotinoids chemistry.chemical_compound Cricetulus Imidacloprid Cricetinae Pyrethrins medicine Animals Environmental Chemistry chemistry.chemical_classification Ivermectin Glutathione peroxidase Imidazoles General Medicine Glutathione Chlorfenapyr Nitro Compounds Pollution Molecular biology chemistry Biochemistry Abamectin Micronucleus Genotoxicity Mutagens |
| Zdroj: | Environmental Science and Pollution Research. 22:17041-17052 |
| ISSN: | 1614-7499 0944-1344 |
| DOI: | 10.1007/s11356-015-4927-3 |
| Popis: | The cytotoxicity and genotoxicity of abamectin, chlorfenapyr, and imidacloprid have been evaluated on the Chinese hamster ovary (CHOK1) cells. Neutral red incorporation (NRI), total cellular protein content (TCP), and methyl tetrazolium (MTT) assays were followed to estimate the mid-point cytotoxicity values, NRI50, TCP50, and MTT50, respectively. The effects of the sublethal concentration (NRI25) on glutathione S-transferase (GST), glutathione reductase (GRD), glutathione peroxidase (GPX), and total glutathione content have been evaluated in the presence and absence of reduced glutathione (GSH), vitamin C, and vitamin E. The genotoxicity was evaluated using chromosomal aberrations (CA), micronucleus (MN) formation, and DNA fragmentation techniques in the presence and absence of the metabolic activation system, S9 mix. Abamectin was the most cytotoxic pesticide followed by chlorfenapyr, while imidacloprid was the least cytotoxic one. The glutathione redox cycle components were altered by the tested pesticides in the absence and presence of the tested antioxidants. The results of genotoxicity indicate that abamectin, chlorfenapyr, and imidacloprid have potential genotoxic effects on CHOK1 cells under the experimental conditions. |
| Databáze: | OpenAIRE |
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