Antitumor Activity with the HSV-tk-gene-modified Cell Line PA-1-STK in Malignant Mesothelioma
| Autor: | Scott M. Freeman, Dinghua Lei, Ann Weinacker, Peng Ye, Jay K. Kolls, Paul Schwarzenberger, Chris Theodossiou, Warren R. Summer |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Mesothelioma Pulmonary and Respiratory Medicine Ganciclovir Pathology medicine.medical_specialty Genetic enhancement Clinical Biochemistry Cell Thymidine Kinase Mice HSV-Tk Gene Tumor Cells Cultured medicine Animals Humans Simplexvirus Molecular Biology business.industry Genetic Therapy Cell Biology medicine.disease In vitro medicine.anatomical_structure Thymidine kinase Cell culture Mice Inbred CBA Cancer research business medicine.drug |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 19:333-337 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb.19.2.3123 |
| Popis: | Malignant mesothelioma (MM) is a thoracic malignancy that is increasing in incidence. Since it is uniformly fatal and kills by local spread, investigators have proposed that MM is a good target for novel treatment approaches, such as gene therapy. We hypothesized that delivery of the HSV-tk gene, using gene-modified tumor cells (PA-1-STK cells), would result in an antitumor effect after treatment with ganciclovir. In in vitro mixing experiments, we found that PA-1-STK cells killed both mouse and human mesothelioma cells in a dose-dependent manner. Moreover, we found that PA-1-STK cells also prolonged survival of mice with MM when the percentage of total tumor cells was high (70%), but observed no survival benefit when the percentage of PA-1-STK cells was low (30%). These data support the rationale for a cell-based gene therapy approach to MM. |
| Databáze: | OpenAIRE |
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