Isotope Coded Labeling for Accelerated Protein Interaction Profiling using MS
| Autor: | Jan Grünewald, Weijia Ou, John D. Venable, Sanjay Agarwalla, Caitlin Steckler, Ansgar Brock |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Chromatography Binding Sites Isotope Extramural Chemistry Proteins Computational biology Tandem mass tag Multiplexing Article Gas Chromatography-Mass Spectrometry Protein Structure Secondary Analytical Chemistry Surface mapping Protein structure Isotope Labeling Protein Interaction Mapping Profiling (information science) |
| Popis: | Protein interaction surface mapping using MS is widely applied but comparatively resource-intensive. Here, a workflow adaptation for use of isotope-coded tandem mass tags for the purpose is reported. The key benefit of improved throughput derived from sample acquisition multiplexing and automated analysis is shown to be maintained in the new application. Mapping of the epitopes of two monoclonal antibodies on their respective targets serves to illustrate the novel approach. We conclude that the approach enables mapping of interactions by MS at significantly larger scales than hereto possible. |
| Databáze: | OpenAIRE |
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