Online acetylcholinesterase inhibition evaluation by high-performance liquid chromatography-mass spectrometry hyphenated with an immobilized enzyme reactor
| Autor: | Ye Yuan, Martine Bergaentzlé, Minjie Zhao, Eric Marchioni, Ludivine Riffault-Valois, Saïd Ennahar |
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| Přispěvatelé: | Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2019 |
| Předmět: |
Bioanalysis
Immobilized enzyme reactor Immobilized enzyme medicine.drug_class 010402 general chemistry 01 natural sciences Biochemistry High-performance liquid chromatography Online Systems Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Bioreactors Limit of Detection Alkaloid medicine [CHIM]Chemical Sciences Huperzine A Chromatography High Pressure Liquid Chromatography Chemistry Elution Galantamine Plant Extracts 010401 analytical chemistry Organic Chemistry Bioanalytical device Reproducibility of Results MS General Medicine Enzymes Immobilized Acetylcholinesterase Acetylcholine 0104 chemical sciences Acetylcholinesterase inhibitor Tacrine Lycoris Cholinesterase Inhibitors medicine.drug |
| Zdroj: | Journal of Chromatography A Journal of Chromatography A, Elsevier, 2020, 1609, pp.460506. ⟨10.1016/j.chroma.2019.460506⟩ |
| ISSN: | 1873-3778 0021-9673 |
| DOI: | 10.1016/j.chroma.2019.460506⟩ |
| Popis: | International audience; A high-performance liquid chromatography-mass spectrometry technique hyphenated on-line with an immobilized enzyme reactor (IMER) was developed by the use of 3 known acetylcholinesterase (AChE) inhibitors (galanthamine, huperzine A and tacrine). This bioanalytical device allows qualitative comparison of the inhibitory strengths of AChE inhibitors. The AChE inhibitory strengths were evaluated and compared by the corresponding acetylcholine peak areas (mass signal) obtained after a chromatographic separation and the elution through the IMER. Only one injection of the analytes is needed to get this comparative analysis. This bioanalytical device was then applied to the extract of a natural plant, Lycoris radiata, which is known to contain AChE inhibitors such as galanthamine and lycoramine. Aside from the demonstration of the inhibitory activity of the two known AChE inhibitors, the AChE inhibitory activity of another compound (dihydro-latifaliumin C) was revealed. This is the first report describing the AChE inhibitory activity of this compound. (C) 2019 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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