CD57+ T cells augment IFN-γproduction in a one-way mixed lymphocyte reaction and their expansion after stem cell transplantation in paediatric patients
| Autor: | T. Kaneko, Isao Sekine, K. Kogawa, Yuji Koike, Hoshio Hiraide, S. Fujitsuka, Shuhji Seki, Takashi Ohkawa |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Cytotoxicity Immunologic Risk Isoantigens Immunology Graft vs Host Disease chemical and pharmacologic phenomena Biology Transplantation Autologous Natural killer cell Interferon-gamma Interleukin 21 CD57 Antigens Immune system T-Lymphocyte Subsets Clinical Studies medicine Humans Transplantation Homologous Immunology and Allergy Cytotoxic T cell Lymphocyte Count Child Cells Cultured Age Factors T lymphocyte Th1 Cells Haematopoiesis medicine.anatomical_structure Child Preschool Endothelium Vascular Lymphocyte Culture Test Mixed Stem cell CD8 Stem Cell Transplantation |
| Zdroj: | Clinical and Experimental Immunology. 130:162-168 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.2002.01958.x |
| Popis: | SummaryTo clarify the immune response of CD57+ T cells (most of them are CD8+) in peripheral blood (PB) against alloantigens in order to elucidate the T helper 1 (Th 1) immune response, we assessed the role of CD57+ T cells in IFN-γ (one of the representative Th 1 cytokines) production in a one-way mixed lymphocyte reaction (MLR). In this study, we showed that CD57+ T cells in responder cells were essential for effective IFN-γ production in allogeneic MLR due partly to the augmentation of the alloresponse of regular T cells. Furthermore, IFN-γ production in MLR correlated with the proportions of CD57+ T cells in PB regardless of the responders’ age. We also showed that the extent of the expansion of CD57+ T cells in paediatric patients after haematopoietic stem cell transplantation (HSCT) was markedly lower than that in adult patients. In addition, CD57+ T cells purified and activated with a combination of cytokines showed a greater cytotoxicity than regular T cells against human umbilical vein endothelial cells. Because IFN-γ production in one-way MLR is a useful predictor of graft-versus-host disease (GVHD), especially in the acute phase that occurs after allogeneic HSCT, our findings suggested that CD57+ T cells play a role in the development of GVHD and thus may explain the reason as to why a higher donor age is associated with an increased risk of developing GVHD while, in addition, the incidence of severe GVHD in paediatric patients is lower than that in adult patients. |
| Databáze: | OpenAIRE |
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